文献类型： 外文期刊
作者： Wang, Xiaolei 1 ; Yang, Zhiyuan 1 ; Wang, Xiuqing 2 ; Duan, Huijuan 1 ; Liu, Lixin 1 ; Cheng, Huimin 1 ; Yang, Chenghu 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Anim Husb & Vet Med, Beijing 100097, Peoples R China
2.South Dakota State Univ, Brookings, SD 57007 USA
3.China Inst Vet Drug Control, Beijing 100081, Peoples R China
关键词： Tembusu virus; hemagglutination inhibition antigen; hemagglutination inhibition test
期刊名称：AVIAN DISEASES （ 影响因子：1.577； 五年影响因子：1.915 ）
ISSN： 0005-2086
年卷期： 2019 年 63 卷 2 期
页码：
收录情况： SCI
摘要： The HB strain of duck Tembusu virus (DTMUV) propagated in the brains of newborn mice was used to prepare antigens for use in the hemagglutination inhibition (HI) test. Results showed that such prepared antigens are highly specific to the serum samples derived from DTMUV-infected animals. No spurious hemagglutination reactions against serum samples specific to avian influenza virus H5, H7, H9 subtypes, Newcastle disease virus, egg drop syndrome virus, duck plague virus, and duck hepatitis A virus were observed. The HI test can detect specific antibodies in the serum samples as early as day 4 after experimental infection of ducks with DTMUV. When compared to a virus neutralization test, the sensitivity is 100%. Overall, the HI test developed is highly specific to DTMUV and can be used in clinical diagnosis of diseases and in vaccine studies to monitor the kinetics of antibody response.
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