文献类型： 外文期刊
作者： Ren, Xiao 1 ; Wang, Xiaolei 2 ; Zhang, Shan 1 ; Gao, Xintao 3 ; Fang, Lichun 1 ; Wang, Xixi 1 ; Lin, Weidong 1 ; Jia, Hon 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Inst Anim Husb & Vet Med, Beijing 100193, Peoples R China
3.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China
关键词： pUC18-CpG; adjuvant; duck Tembusu virus; inactivated vaccine
期刊名称：VIRUSES-BASEL （ 影响因子：5.048； 五年影响因子：5.127 ）
ISSN：
年卷期： 2020 年 12 卷 2 期
页码：
收录情况： SCI
摘要： Duck Tembusu virus (DTMUV) is an emerging pathogenic flavivirus responsible for massive economic losses in the duck industry. However, commercially inactivated DTMUV vaccines have been ineffective at inducing protective immunity in ducks. The widely used adjuvant cytosine-phosphate-guanine oligodeoxynucleotides (CpG ODNs) reportedly improve humoral and cellular immunities in animal models. However, its effectiveness in DTMUV vaccines requires validation. Here, we assessed the protective efficacy of pUC18-CpG as an adjuvant in an inactivated live DTMUV vaccine in ducks. Our results revealed that the serum hemagglutination inhibition (HI) antibody titers, positive rates of anti-DTMUV antibodies, the concentration of serum cytokines, and protection efficacy were significantly increased in ducks immunized with pUC18-CpG compared to that in the control group. Moreover, ducks immunized with a full vaccine dose containing a half dose of antigen supplemented with 40 mu g of pUC18-CpG exhibited the most potent responses. This study suggests that pUC18-CpG is a promising adjuvant against DTMUV, which might prove effective in treating other viral diseases in waterfowl.
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