文献类型： 外文期刊

作者： Ma Zhen ¹ ; Zhuang Hui-Sheng ¹ ; Yang Guang-Xin ² ;

作者机构： 1.Shanghai Jiao Tong Univ, Sch Environm Sci & Engn, Shanghai 200240, Peoples R China

2.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China

关键词： Tetrabromobisphenol A; Real-time immuno-polymerase chain reaction; Immunoassay; Functionalized nanoprobes; PM2.5 samples

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）

ISSN： 0253-3820

年卷期： 2019 年 47 卷 4 期

页码：

收录情况： SCI

摘要： Tetrabromobisphenol A (TBBPA), a type of brominated flame retardant, is widely distributed in the environment. In this work, different TBBPA antigens and polyclonal anti-TBBPA antibody (pAb-TBBPA) were prepared, and on the basis of this, an ultrasensitive real-time immuno-polymerase chain reaction for TBBPA detection using functionalized nanoprobes (FNPs-IPCR) was developed. Gold nanoparticles modified with thiol-capped DNA and pAb-TBBPA were prepared as antibody-DNA conjugates, and the signal intensity of the immunoassay was greatly enhanced. Under optimal conditions, the proposed immunoassay was used to detect TBBPA with a linearity ranging from 1 pg L-1 to 10 ng L-1, and the limit of detection was 0.62 pg L-1. The recovery was 89.2%-107.4% and the coefficient of variation was 3.17%-7.73%. The FNPs-IPCR assay was highly selective and sensitive. Finally, the detection results of TBBPA in PM2.5 samples were consistent with those from high performance liquid chromatography, indicating that the FNPs-IPCR method is suitable for trace TBBPA screening in environmental samples.