文献类型： 外文期刊

作者： Yu, Yanjun ¹ ; Wu, Shenjie ³ ; Nowak, Jacqueline ⁴ ; Wang, Guangda ¹ ; Han, Libo ¹ ; Feng, Zhidi ¹ ; Mendrinna, Amelie ⁴ ;

作者机构： 1.Chinese Acad Sci, Acad Seed Design, Inst Microbiol, State Key Lab Plant Genom, Beijing, Peoples R China

2.Univ Chinese Acad Sci, Beijing, Peoples R China

3.Shanxi Acad Agr Sci, Cotton Res Inst, Yucheng, Peoples R China

4.Univ Melbourne, Sch Biosci, Melbourne, Vic, Australia

5.Max Planck Inst Mol Plant Physiol, Syst Biol & Math Modeling, Potsdam, Germany

6.Univ Potsdam, Inst Biochem & Biol, Bioinformat, Potsdam, Germany

期刊名称：NATURE PLANTS （ 影响因子：15.793； 五年影响因子：17.349 ）

ISSN： 2055-026X

年卷期： 2019 年 5 卷 5 期

页码：

收录情况： SCI

摘要： Cotton (Gossypium hirsutum) fibres consist of single cells that grow in a highly polarized manner, assumed to be controlled by the cytoskeleton(1-3). However, how the cytoskeletal organization and dynamics underpin fibre development remains unexplored. Moreover, it is unclear whether cotton fibres expand via tip growth or diffuse growth(2-4). We generated stable transgenic cotton plants expressing fluorescent markers of the actin and microtubule cytoskeleton. Live-cell imaging revealed that elongating cotton fibres assemble a cortical filamentous actin network that extends along the cell axis to finally form actin strands with closed loops in the tapered fibre tip. Analyses of F-actin network properties indicate that cotton fibres have a unique actin organization that blends features of both diffuse and tip growth modes. Interestingly, typical actin organization and endosomal vesicle aggregation found in tip-growing cell apices were not observed in fibre tips. Instead, endomembrane compartments were evenly distributed along the elongating fibre cells and moved bi-directionally along the fibre shank to the fibre tip. Moreover, plus-end tracked microtubules transversely encircled elongating fibre shanks, reminiscent of diffusely growing cells. Collectively, our findings indicate that cotton fibres elongate via a unique tip-biased diffuse growth mode.