Cytochrome P450 monooxygenases-mediated sex-differential spinosad resistance in house flies Musca domestica (Diptera: Muscidae)
文献类型： 外文期刊
作者： Zhang, Yi 1 ; Wang, Yanyan 1 ; Ma, Zhuo 1 ; Zhai, Dai 1 ; Gao, Xiwu 1 ; Shi, Xueyan 1 ;
作者机构： 1.China Agr Univ, Dept Entomol, Beijing 100193, Peoples R China
2.Zhejiang Acad Agr Sci, Inst Qual & Stand Agroprod, State Key Lab Breeding Base Zhejiang Sustainable, MOA Key Lab Pesticide Residue Detect, Hangzhou 310021, Zhejiang, Peoples R China
3.SIPO, Patent Off, Patent Examinat Cooperat Jiangsu Ctr, Suzhou 215163, Jiangsu, Peoples R China
关键词： Musca domestica; Spinosad resistance; Sex-differential; Cytochrome P450 monooxygenases
期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：3.963； 五年影响因子：4.454 ）
ISSN： 0048-3575
年卷期： 2019 年 157 卷
页码：
收录情况： SCI
摘要： Females developed notably higher resistance than males in a spinosad-resistant house fly strain, however, resistance factors responsible for this phenomenon are poorly understood. In this study, the potential role of cytochrome P450 monooxygenases involved in the sex-differential spinosad resistance in house flies was investigated, using a susceptible strain (SSS) and a spinosad resistant near-isogenic line (N-SRS). Combination of the synergism of spinosad by PBO and increased cytochrome P450 monooxygenase activity in the N-SRS strain implied that cytochrome P450 monooxygenases contributed to spinosad resistance in house flies. Transcriptional levels of eight P450 genes related to insecticide resistance in two genders of the SSS and N-SRS strain were separately evaluated by quantitative real-time PCR. Notably, compared with the corresponding gender of susceptible SSS house flies, CYP4G2 and CYP6A5v2 were overexpressed in resistant N-SRS females, while the expression of these two P450 genes was significantly decreased in resistant N-SRS males. Furthermore, by measuring the expression of CYP4G2 and CYP6A5v2 in female and male house fly populations with different spinosad resistance levels, which were generated from a series of genetic crosses, the genetic linkage between spinosad resistance and P450 gene expression was analyzed. It was found that with increased spinosad resistance, CYP4G2 and CYP6A5v2 were up-regulated in females, while both of them were down-regulated in males, and this suggested their involvement in the female-linked spinosad resistance of house flies. Taken together, our results provide valuable insight into the involvement of cytochrome P450 monooxygenases in the sex-differential spinosad resistance in house flies.
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