Transcriptomic Profiling Highlights Metabolic and Biosynthetic Pathways Involved in In Vitro Flowering in Anoectochilus roxburghii (Wall.) Lindl.
文献类型： 外文期刊
作者： Yu, Shuisheng 1 ; Liu, Julian 1 ; Cai, Chenchen 1 ; Zhang, Yi 1 ; Fu, Shuangbin 2 ; Yang, Yanping 2 ; Zhou, Zhuang 2 ; Ying, Zhen 2 ;
作者机构： 1.Zhejiang Jiulongshan Natl Nat Reserve Management C, Lishui 323312, Peoples R China
2.Zhejiang Inst Subtrop Crops, Wenzhou 325005, Peoples R China
3.Zhejiang Acad Agr Sci, Innovat Ctr Chinese Med Crops, Hangzhou 310021, Peoples R China
关键词： flower; RNA-seq; metabolic pathways; biosynthetic pathways; DNA replication
期刊名称：GENES （ 影响因子：2.8； 五年影响因子：3.2 ）
ISSN：
年卷期： 2025 年 16 卷 2 期
页码：
收录情况： SCI
摘要： Background: Tissue culture is one of the most important methods for propagating orchids. Notably, many orchid seedlings exhibit autonomous flowering during the cultivation process. To explore the underlying mechanism, Anoectochilus roxburghii (Wall.) Lindl., an orchid that spontaneously forms in vitro flowers, was analyzed in this study. Methods: Bud samples at the early, middle, and fully open stages were collected for transcriptome sequencing, followed by differential expression, trend, enrichment and protein-protein interaction (PPI) network analyses. Results: Differential gene expression analysis identified 2364, 4137, and 6522 differentially expressed genes (DEGs) in the early vs. middle, middle vs. open, and early vs. open comparisons, respectively. These DEGs were significantly enriched in various metabolic and biosynthetic pathways, particularly in ko01100 (metabolic pathways). PPI network analysis further identified hub genes, including MCM3, MCM4, and MCM7, which are associated with DNA replication, and CURL3, which is linked to plant hormone signal transduction pathways. Conclusion: Our findings provide novel insights into the molecular mechanisms driving in vitro flowering in A. roxburghii, highlighting the importance of metabolic and biosynthetic process signaling in this unique developmental transition. These results provide valuable resources for future studies on orchid propagation and floral development.
- 相关文献
作者其他论文 更多>>
-
Characteristics and Phylogenetic Analysis of the Complete Plastomes of Anthogonium gracile and Eleorchis japonica (Epidendroideae, Orchidaceae)
作者：Gao, Xuyong;Chen, Yuming;Li, Minghe;Gao, Xuyong;Chen, Yuming;Zhou, Zhuang;Xu, Xiaowei;Chen, Hongjiang;Xing, Bingcong;Pan, Jianli
关键词：Arethusinae; Anthogonium gracile; Eleorchis japonica; plastid genome; phylogenetic analysis
-
Analysis of lettuce transcriptome reveals the mechanism of different light/dark cycle in promoting the growth and quality
作者：Dai, Mengdi;Tan, Xiangfeng;Ye, Ziran;Chen, Xuting;Kong, Dedong;Zhang, Yi;Ruan, Yunjie;Ruan, Yunjie;Ma, Bin;Ma, Bin
关键词：Lactuca sativa; transcriptome; L/D cycle; growth; quality
-
A hyperspectral deep learning attention model for predicting lettuce chlorophyll content
作者：Ye, Ziran;Tan, Xiangfeng;Dai, Mengdi;Chen, Xuting;Zhong, Yuanxiang;Kong, Dedong;Zhang, Yi;Ruan, Yunjie;Ruan, Yunjie
关键词：Lettuce; Chlorophyll content; Beep learning; Hyperspectral
-
The Complete Chloroplast Genome of an Epiphytic Leafless Orchid, Taeniophyllum complanatum: Comparative Analysis and Phylogenetic Relationships
作者：Zhou, Zhuang;Chen, Jinliao;Wang, Fei;Wu, Xiaopei;Liu, Zhongjian;Peng, Donghui;Lan, Siren;Zhou, Zhuang
关键词：chloroplast genome; Taeniophyllum complanatum; codon usage; SSR and repeat analysis; phylogenetic analysis; epiphytic leafless orchid
-
Using widely targeted metabolomics profiling to explore differences in constituents of three Bletilla species
作者：Xu, Wan;Huang, Jian;Wang, Peilong;Yang, Yanping;Fu, Shuangbin;Ying, Zhen;Zhou, Zhuang
关键词：Bletilla striata; Bletilla ochracea; Bletilla formosana; Metabolites; Chinese traditional medicine
-
Characterization and Comparative Analysis of the Complete Plastomes of Five Epidendrum (Epidendreae, Orchidaceae) Species
作者：Zhao, Zhuang;Zeng, Meng-Yao;Wu, Yu-Wei;Li, Jin-Wei;Zhou, Zhuang;Liu, Zhong-Jian;Li, Ming-He;Zhou, Zhuang;Liu, Zhong-Jian;Li, Ming-He
关键词：Epidendrum; plastid genome; mutational hotspots; phylogenetic analysis
-
Soil bacterial community composition and diversity respond to soil environment in rooftop agricultural system
作者：Dai, Mengdi;Tan, Xiangfeng;Ye, Ziran;Li, Bojun;Chen, Xuting;Kong, Dedong;Zhang, Yi;Kong, Dedong
关键词：Rooftop farm; Microcirculation system; Soil environment; Bacterial community; Co-occurrence network
