Molecular cloning, characterization, and expression profiling analysis of Cry toxin receptor genes from sugarcane shoot borer Chilo infuscatellus (Snellen)
文献类型： 外文期刊
作者： Wang, Jin-da 1 ; Zhang, Jia-song 1 ; Guo, Yan-Fang 1 ; Chen, Li-fei 1 ; Wang, Fa-Lv 1 ; Huang, Mei-ting 1 ; Gao, San-j 1 ;
作者机构： 1.Fujian Agr & Forestry Univ, Natl Engn Res Ctr Sugarcane, Fuzhou 350002, Fujian, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China
关键词： Chilo infuscatellus; Cry toxin receptor; Sublethal dosage; Expression profile
期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：3.963； 五年影响因子：4.454 ）
ISSN： 0048-3575
年卷期： 2019 年 157 卷
页码：
收录情况： SCI
摘要： The sugarcane shoot borer Chilo infuscatellus (Snellen) is known for causing severe damage to sugarcane yield in China. Methods have been developed to control this pest, including Cry toxin pesticide and transgenic Bt plants. In order to investigate the molecular mechanism of the Cry toxin binding process and provide a basis for understanding the insect's resistance mechanism, we used a high throughput sequencing platform to perform a de novo transcriptome assembly across different larval developmental stages and analyzed Cry toxin receptors based on our assembled transcripts. We cloned twelve Cry toxin receptor genes including 1 cadherin (Cad), 7 aminopeptidase-Ns (APNs), 3 alkaline phosphatases (ALPs), and 1 ATP-binding cassette transporter subfamily C2 (ABCC2), and three of them with full length. The sublethal dosage of Cry1Ac toxin was applied to sugarcane shoot borer and identified some Cry toxin receptor genes that were significantly induced after 48 h of exposure. Furthermore, quantitative RT-PCR was conducted to detect the expression profiles of these genes. Our transcriptome sequence data provided a valuable molecular resource for further study and the identified Cry toxin receptor data gave insights for improved research into the mechanism of Bt resistance.
- 相关文献
作者其他论文 更多>>
-
Overexpression of CYP6CX4 contributing to field-evolved resistance to flupyradifurone, one novel butenolide insecticide, in Bemisia tabaci from China
作者：Zhang, Qinghe;Tang, Juan;Gao, Bingli;Qu, Cheng;Luo, Chen;Wang, Ran;Tang, Juan;Wang, Jinda
关键词：Bemisia tabaci; Flupyradifurone; Cytochrome P450
-
Knockdown of one cytochrome P450 gene CYP6DW4 increases the susceptibility of Bemisia tabaci to dimpropyridaz, a novel pyridazine pyrazolecarboxamide insecticide
作者：Tang, Juan;Su, Qi;Tang, Juan;Zhang, Qinghe;Qu, Cheng;Luo, Chen;Wang, Ran;Tang, Juan;Zhang, Qinghe;Qu, Cheng;Luo, Chen;Wang, Ran
关键词：Bemisia tabaci; Dimpropyridaz; Insecticide detoxification; Cytochrome P450 monooxygenase
-
Tetraniliprole resistance in field-collected populations of Tuta absoluta (Lepidoptera: Gelechiidae) from China: Baseline susceptibility, cross-resistance, inheritance, and biochemical mechanism
作者：Qu, Cheng;Yao, Jiaqi;Luo, Chen;Wang, Ran;Yao, Jiaqi;Huang, Jianlei;Che, Wunan;Fang, Yong;Fang, Yong
关键词：Tuta absoluta; Tetraniliprole; Cross -resistance; Synergism; Metabolic enzyme; Mode of inheritance
-
Tetraniliprole risk assessment: Unveiling a hidden threat for managing a generalist herbivore
作者：Qu, Cheng;Li, Yunyi;Zhan, Qianyuan;Luo, Chen;Wang, Ran;Qu, Cheng;Wang, Ran;Wang, Jinda;Guedes, Raul Narciso C.
关键词：Tobacco cutworm; Realized heritability; Baseline of susceptibility; Mode of inheritance; Biochemical mechanism; Transgenerational hormesis
-
Risk assessment, cross-resistance, biochemical mechanism and fitness cost of tetraniliprole resistance in the tomato pinworm Tuta absoluta
作者：Qu, Cheng;Yao, Jiaqi;Zhan, Qianyuan;Zhang, Daofeng;Li, Yunyi;Wang, Ran;Qu, Cheng;Yao, Jiaqi;Zhan, Qianyuan;Zhang, Daofeng;Li, Yunyi;Wang, Ran;Yao, Jiaqi;Huang, Jianlei
关键词：Tuta absoluta; Tetraniliprole; Risk assessment; Cross -resistance; Biochemical mechanism; Fitness cost
-
Baseline of susceptibility, risk assessment, biochemical mechanism, and fitness cost of resistance to dimpropyridaz, a novel pyridazine pyrazolecarboxamide insecticide, in Bemisia tabaci from China
作者：Tang, Juan;Qu, Cheng;Zhan, Qianyuan;Zhang, Daofeng;Luo, Chen;Wang, Ran;Tang, Juan;Qu, Cheng;Zhan, Qianyuan;Zhang, Daofeng;Luo, Chen;Wang, Ran;Wang, Jinda
关键词：Bemisia tabaci; Dimpropyridaz; Baseline of susceptibility; Risk assessment; Fitness cost; Detoxifying enzymes
-
Insecticide Resistance and Its Management in Two Invasive Cryptic Species of Bemisia tabaci in China
作者：Wang, Qian;Luo, Chen;Wang, Ran
关键词：Bemisia tabaci; invasive whiteflies; insecticide resistance; pest control; resistance management
