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Identification and Characterization of ATP/ADP Isopentenyltransferases (ATP/ADP PpIPTs) Genes in Peach

文献类型： 外文期刊

作者： Li, Min-ji 1 ; Wei, Qin-ping 1 ; Peng, Fu-tian 2 ; Yu, Wen 2 ; Luo, Jing-jing 2 ; Zhao, Yong-fei 2 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Acad Forestry & Pomol Sci, Minist Agr, Key Lab Urban Agr North China, Beijing 100093, Peoples R China

2.Shandong Agr Univ, State Key Lab Crop Biol, Coll Hort Sci & Engn, Tai An 271018, Shandong, Peoples R China

关键词： ATP; ADP PpIPTs; Peach (Prunus persica); Fruit; Tissue-specific expression; Plant hormone; Nitrate

期刊名称：JOURNAL OF PLANT GROWTH REGULATION （ 影响因子：4.169； 五年影响因子：4.038 ）

ISSN： 0721-7595

年卷期： 2019 年 38 卷 2 期

页码：

收录情况： SCI

摘要： ATP/ADP isopentenyltransferase (IPTs) genes encode key enzymes involved in cytokinin synthesis. In this study, the functions of ATP/ADP PpIPTs in peach were investigated. According to the genome sequence, we have found and verified that there are four members of this gene family in peach, namely, PpIPT1, PpIPT3, PpIPT5, and PpIPT7. Overexpression of each of these genes in Arabidopsis resulted in increased levels of cytokinins in the transgenic plants, confirming their roles in cytokinin synthesis. Numerous altered phenotypes were observed in the transgenic plants, including vigorous growth and enhanced salt resistance. ATP/ADP PpIPTs were expressed in tissues throughout the plant, but the expression patterns differed between the genes. Only PpIPT3 was upregulated within 2h after the application of nitrate to N-deprived peach seedlings, and the increase was resistant to pre-treatment of a specific nitrate metabolism inhibitor. Results showed that ATP/ADP PpIPT expression levels decreased significantly in pulp within 2weeks after flowering and remained low. However, pulp cytokinin levels were quite high during this time. Only PpIPT5 in seed increased significantly within 2weeks after flowering, which was consistent with cytokinin levels during early fruit development, suggesting that PpIPT5 in seed is the key gene for cytokinin biosynthesis during early fruit development. ATP/ADP PpIPT expression also increased significantly during later fruit development in seed.

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