The transcription factor MhZAT10 enhances antioxidant capacity by directly activating the antioxidant genes MhMSD1, MhAPX3a and MhCAT1 in apple rootstock SH6 (Malus honanensis x M. domestica)
文献类型： 外文期刊
作者： Yang, Qian 1 ; Liu, Yan 1 ; Zhou, Jia 1 ; Li, Min-Ji 1 ; Yang, Yu-Zhang 1 ; Wei, Qin-Ping 1 ; Zhang, Jun-Ke 1 ; Li, Xing-Liang 1 ;
作者机构： 1.Inst Forestry & Pomol, Beijing Acad Agr & Forestry Sci, Key Lab Biol & Genet Improvement Hort Crops North, Minzhuang Rd 12, Beijing 100093, Peoples R China
关键词： antioxidation; apple; osmotic stress; signal transduction; transcription factor
期刊名称：TREE PHYSIOLOGY （ 影响因子：3.5； 五年影响因子：4.1 ）
ISSN： 0829-318X
年卷期： 2024 年 44 卷 7 期
页码：
收录情况： SCI
摘要： Stress tolerance in apple (Malus domestica) can be improved by grafting to a stress-tolerant rootstock, such as 'SH6' (Malus honanensis x M. domestica 'Ralls Genet'). However, the mechanisms of stress tolerance in this rootstock are unclear. In Arabidopsis (Arabidopsis thaliana), the transcription factor ZINC FINGER OF ARABIDOPSIS THALIANA 10 is a key component of plant tolerance to multiple abiotic stresses and positively regulates antioxidant enzymes. However, how reactive oxygen species are eliminated upon activation of ZINC FINGER OF ARABIDOPSIS THALIANA 10 in response to abiotic stress remains elusive. Here, we report that MhZAT10 in the rootstock SH6 directly activates the transcription of three genes encoding the antioxidant enzymes MANGANESE SUPEROXIDE DISMUTASE 1 (MhMSD1), ASCORBATE PEROXIDASE 3A (MhAPX3a) and CATALASE 1 (MhCAT1) by binding to their promoters. Heterologous expression in Arabidopsis protoplasts showed that MhMSD1, MhAPX3a and MhCAT1 localize in multiple subcellular compartments. Overexpressing MhMSD1, MhAPX3a or MhCAT1 in SH6 fruit calli resulted in higher superoxide dismutase, ascorbate peroxidase and catalase enzyme activities in their respective overexpressing calli than in those overexpressing MhZAT10. Notably, the calli overexpressing MhZAT10 exhibited better growth and lower reactive oxygen species levels under simulated osmotic stress. Apple SH6 plants overexpressing MhZAT10 in their roots via Agrobacterium rhizogenes-mediated transformation also showed enhanced tolerance to osmotic stress, with higher leaf photosynthetic capacity, relative water content in roots and antioxidant enzyme activity, as well as less reactive oxygen species accumulation. Overall, our study demonstrates that the transcription factor MhZAT10 synergistically regulates the transcription of multiple antioxidant-related genes and elevates reactive oxygen species detoxification.
- 相关文献
作者其他论文 更多>>
-
Yes-associated protein 1 is essential for maintaining lactation via regulating mammary epithelial cell dynamics and secretion capacity
作者：Lv, Haimiao;Wu, Zihui;Lin, Yuxin;Liu, Miaoyu;Xu, Linghua;Wang, Xiaojie;Sun, Nan;Abdelrahman, Mohamed;Yang, Liguo;Hua, Guohua;Lv, Haimiao;Hua, Guohua;Lv, Haimiao;Hua, Guohua;Xu, Xiaoling;Liu, Yan;Abdel-Shafy, Hamdy;Abdelrahman, Mohamed;Alsaegh, Aiman A.;Ahmed, Ahmed Ezzat;Ahmed, Ahmed Ezzat;Yang, Liguo;Hua, Guohua
关键词：Lactation; Yes-associated protein1(YAP1); Prolactin; Melatonin; Mammary epithelial cells
-
Effects of cloprostenol sodium and carbetocin on synchronous parturition and colostrum composition in large white sows
作者：Gao, Hongmei;Zhang, Longchao;Wang, Dong;Wang, Lixian;Peng, Miaolian;Zhong, Rongzhi;Xue, Zhenhua;Liu, Zhenqiang;Weng, Shiqiao;Liu, Yan;Tian, Jianhui;Tian, Jianhui;Tian, Jianhui
关键词：Cloprostenol sodium; Carbetocin; Large white sows; Farrowing induction; Colostrum
-
Design, Synthesis and Bioactivity of Novel 2-Phenylethanol Ester Derivatives Containing Monoterpene Fragments
作者：Pan, Shixiang;Liu, Yan;Shi, Zhuo;Lu, Xingxing;Chen, Chen;Yang, Xinling;Qin, Yaoguo;Qu, Cheng
关键词：2-phenylethanol; odorant binding protein; repel aphid; attract ladybug; eco-friendliness
-
Alterations to the vaginal microbiota and their correlation with serum pro-inflammatory cytokines in post-weaning sows with endometritis
作者：Xian, Tingting;Cao, Xin;Xian, Tingting;Liu, Yan;Feng, Tao;Xian, Tingting;Liu, Yan;Feng, Tao
关键词：Post-weaning sows; Endometritis; Cytokines; 16S rDNA sequencing; Vaginal mucus microflora
-
Editing Plant Volatile Isoeugenol for Integrated Aphid Management: Discovering Ecofriendly Insect Behavioral Regulators with Push-Pull Activity Targeting Odorant-Binding Proteins
作者：Liu, Yan;Chen, Chen;Zhang, Yimeng;Huang, Jiachuan;Pan, Shixiang;Yang, Zhaokai;Shi, Zhuo;Yang, Xinling;Qu, Cheng;Huang, Yiwen;Qin, Yaoguo;Huang, Yiwen;Qin, Yaoguo;Li, Xuesheng
关键词：insect behavioral regulator; "push-pull"strategy; odorant-binding proteins; ecofriendlybotanical derivatives; molecular docking; densityfunctional theory
-
Effects of long-acting recombinant porcine FSH on reproductive performance and production efficiency of gilts and weaned sows
作者：Zhang, Jiawei;Zhang, Jiawei;Shi, Lei;Huang, Zheng;Cheng, Shiwen;Han, Guo;Xiong, Jiansheng;Luo, Haoshu;Bai, Jiahua;Liu, Yan;Xie, Shuyu;Chen, Dongmei;Xie, Shuyu;Xie, Shuyu
关键词：Estrous synchronization; Follicular development; Recombinant FSH; Gilts; Weaned sows
-
Identification of the Na+ transporter gene MdNCL4 in response to high salinity stress in apple rootstock (Malus domestica (Suckow) Borkh.)
作者：Guo, Xinxin;Shi, Tianle;Zhou, Jia;Yang, Yuzhang;Li, Minji;Zhang, Junke;Li, Xingliang;Guo, Xinxin;Shi, Tianle;Zhou, Jia;Yang, Yuzhang;Li, Minji;Zhang, Junke;Li, Xingliang
关键词：Apple; salt stress; Na+ transport; gene expression; Na+/Ca2+ exchanger
