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P,N Codoped carbon dots as an efficient "off-on" fluorescent probe for lipoic acid detection and its cellular dual-color imaging

文献类型： 外文期刊

作者： Hu, Yuefang 1 ; Lin, Liyun 3 ; Li, Jinfang 1 ; Ye, Jianzhi 3 ;

作者机构： 1.Hezhou Univ, Coll Mat & Chem Engn, Hezhou 542899, Guangxi, Peoples R China

2.Guangxi Normal Univ, Coll Chem & Pharmaceut Sci, Guilin 541004, Guangxi, Peoples R China

3.Chinese Acad Trop Agr Sci, Prod Proc Res Inst, Zhanjiang 524001, Guangdong, Peoples R China

关键词： Codoped carbon dots; LA detection; Fluorescent probe; Cell imaging

期刊名称：ANALYTICAL AND BIOANALYTICAL CHEMISTRY （ 影响因子：4.142； 五年影响因子：3.863 ）

ISSN： 1618-2642

年卷期： 2019 年 411 卷 16 期

页码：

收录情况： SCI

摘要： A facile single hydrothermal method was developed to synthetize P,N codoped carbon dots (P,N/CDs), which show strong and stable fluorescence, good water solubility, low toxicity and good biocompatibility. Hence, a novel and efficient off-on P,N/CDs fluorescent probe was developed for the highly sensitive detection of lipoic acid (LA) for the first time. The fluorescence of the P,N/CDs was quenched by Cu2+ forming a P,N/CDs-Cu2+ complex, which acted as the off process, but Cu2+ could be removed by LA, due to stronger chelating between LA and Cu2+, forming a more stable complex, which recovered the fluorescence of the P,N/CDs, in order to achieve the on process. Under optimal conditions, the concentration of LA and the increased fluorescence intensity of the P,N/CDs-Cu2+ complex displayed a good linear relationship within the range of 0.05-28M, with a detection limit (S/N=3) of 0.02M. The established off-on fluorescent probe was successfully applied to the analysis of LA in urine samples. The average recoveries were in the range of 98.3-101.5%, with a relative standard deviations of less than 3.1%. In addition, the P,N/CDs were also successfully applied to cellular dual-color imaging of live T24 cells. The results show that the P,N/CDs have great application potential in clinical diagnosis, bioassay and bioimaging.

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