Effective detection of Lily Symptomless Virus using the reverse transcription loop-mediated isothermal amplification method
文献类型： 外文期刊
作者： Zhao, Xiao 1 ; Du, Yanan 2 ; Zhang, Yongchun 1 ; Liang, Jieling 3 ; Cai, Youming 1 ; Xu, Yan 2 ; Fan, Xiaorui 2 ; Wu, Wen 1 ;
作者机构： 1.Shanghai Acad Agr Sci, 2901 Beidi Rd, Shanghai 201106, Peoples R China
2.Shanghai Normal Univ, Coll Life & Environm Sci, 100 Guilin Rd, Shanghai 200234, Peoples R China
3.Taizhou Univ, Sch Life Sci, 1139 Shifu Rd, Taizhou 317000, Peoples R China
4.Shanghai Acad Agr Sci, Key Lab Agr Genet & Breeding, 2901 Beidi Rd, Shanghai 201106, Peoples R China
关键词： RT-LAMP; Lily; Carlavirus; Virus detection
期刊名称：AUSTRALASIAN PLANT PATHOLOGY （ 影响因子：1.599； 五年影响因子：1.708 ）
ISSN： 0815-3191
年卷期： 2019 年 48 卷 4 期
页码：
收录情况： SCI
摘要： Lily Symptomless Virus (LSV) is prevalent in lily, causing severe economic losses in Lily production. Therefore, it is quite necessary to develop an effective method for detecting LSV. This study aimed to develop a visual Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) method to detect LSV. The optimum condition was 60min at 65 degrees C. The primers designed were highly specific to LSV and did not cross-react with other seven Lily viruses. The sensitivity of RT-LAMP assay for LSV was 0.0001ng/ul. This RT-LAMP method was rapid, simple, highly specific, and sensitive and helped in the reliable visual detection of LSV in Lily plants.
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