Identification of microRNA-21 target genes associated with hair follicle development in sheep
文献类型： 外文期刊
作者： Zhai, Bo 1 ; Zhang, Lichun 1 ; Wang, Chunxin 1 ; Zhao, Zhuo 1 ; Zhang, Mingxin 1 ; Li, Xu 1 ;
作者机构： 1.Jilin Acad Agr Sci, Branch Anim Husb, Gongzhuling, Peoples R China
关键词： microRNA-21; Sheep; Hair follicles
期刊名称：PEERJ （ 影响因子：2.984； 五年影响因子：3.369 ）
ISSN： 2167-8359
年卷期： 2019 年 7 卷
页码：
收录情况： SCI
摘要： Aim. The target molecule regulatory function of microRNA-21 (miR-21) in multiple signalling pathways has become a main focus of genetic and pharmacological regulatory studies of various diseases. The identification of target genes for miRNA-21 in the development of hair follicles can provide new research pathways for the regulation of cell development. Methods. In the present study, eight six-month-old ewes from Super Merino (SM) and Small Tailed Han (STH) sheep breeds were selected. Target prediction and dual-luciferase wild-type and mutant vectors were used to identify the target genes of miR-21. Quantitative reverse transcription polymerase chain reaction (RT-qPCR) and bioinformatics analysis were conducted to analyze the effects of miR-21. Results. The results show that the expressions of CNKSR2, KLF3 and TNPO1 were downregulated by miRNA-21 at rates of 36%, 26% and 48%, respectively. Moreover, there was a significant negative correlation between the expression of miR-21 and the three target genes in sheep with two extreme phenotypes. The expression of microRNA-21in October was significantly lower than that in January and February; while the expression of CNKSR2, KLF3 and TNPO1 in October was higher than that in January and February. Conclusions: These results suggest that CNKSR2, KLF3 and TNPO1 are three newly discovered target genes of miR-21 and might be involved in the effects of miR-21 on hair follicle development.
- 相关文献
作者其他论文 更多>>
-
Two mutations at KRT74 and EDAR synergistically drive the fine-wool production in Chinese sheep
作者：Liang, Benmeng;Bai, Tianyou;Zhao, Yuhetian;Han, Jiangang;He, Xiaohong;Pu, Yabin;Ma, Yuehui;Jiang, Lin;Liang, Benmeng;Liu, Jianfeng;Liang, Benmeng;Liu, Jianfeng;Liang, Benmeng;Bai, Tianyou;Zhao, Yuhetian;Han, Jiangang;He, Xiaohong;Pu, Yabin;Ma, Yuehui;Jiang, Lin;Han, Jiangang;Wang, Chunxin;Liu, Wujun;Ma, Qing;Tian, Kechuan;Tian, Kechuan;Zheng, Wenxin;Liu, Nan
关键词：Chinese fine wool sheep; Whole genome sequencing; Genomic selection signature; Follicle density; Missense; Regulatory
-
Alpha-lipoic acid improves cryopreservation of rooster semen by reducing oxidative stress
作者：Chen, Xiaoxin;Liu, Jianqiang;Liu, Yi;Li, Xu;An, Dingjie;Liu, Xiaohui;Zhang, Lichun
关键词：alpha-lipoic acid; antioxidant capacity; semen freezing; rooster sperm ultramicrostructure
-
The comparison of meat yield, quality, and flavor between small-tailed Han sheep and two crossbred sheep and the verification of related candidate genes
作者：Xiao, Cheng;Liu, Yu;Zhao, Wenjun;Liang, Yingjia;Cui, Chao;Yang, Shaoying;Fang, WenWen;Miao, Lisheng;Yuan, Zhiyu;Zhai, Bo;Zhao, Zhongli;Zhang, Lichun;Ma, Huihai;Jin, Haiguo;Cao, Yang;Xiao, Cheng;Xiao, Cheng;Zhao, Wenjun;Lin, Zihan
关键词：small-tailed Han sheep; crossbred sheep; meat yield; quality; volatile compound; PDK4 gene; TMEM273 gene
-
Development and Immunoprotection of Bacterium-like Particle Vaccine against Infectious Bronchitis in Chickens
作者：Zhang, Pengju;Qiao, Haiying;Hu, Nianzhi;Li, Xintao;Wang, Weixia;Zhang, Lichun;Yang, Tiantian;Cong, Yanlong;Sun, Yixue
关键词：infectious bronchitis virus; S1 subunit; bacterium-like particles; immunoprotective
-
Long non-coding RNA NEAT1 promotes mouse granulosa cell proliferation and estradiol synthesis by sponging miR-874-3p
作者：Zhang, Pengju;Wang, Weixia;Tan, Chengcheng;Liu, Xiaohui;Li, Xintao;Zhang, Lichun;Gao, Jinliang;Lin, Shan;Lin, Guangyu;Li, Xintao;Zhang, Lichun
关键词：long non-coding RNA; nuclear-enriched abundant transcript 1; mouse granulosa cell; apoptosis; estradiol synthesis
-
Effect of Controlling Light on Cashmere Growth and Harmful Gas Parameters in Shanbei White Cashmere Goats
作者：Cui, Wenyuan;Lin, Changlong;Liu, Yuyang;Qiu, Zhixin;Chen, Yulin;Yang, Yuxin;Gao, Wenrui;Wang, Chunxin
关键词：goat; cashmere; light control; photoperiod; harmful gas
-
Effects of short photoperiod on cashmere growth, hormone concentrations and hair follicle development-related gene expression in cashmere goats
作者：Li, Junda;Tian, Guangjie;Wang, Xingtao;Tang, Hongyu;Liu, Yuyang;Guo, Hongran;Chen, Yulin;Yang, Yuxin;Wang, Chunxin;Yang, Yuxin
关键词：Cashmere goat; photoperiod; hair follicles; hormone; gene
