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Enhancement of xylanase expression by Komagataella phaffii through pexophagy inhibition

文献类型： 外文期刊

作者： Liu, Taiyu 1 ; Zhao, Yixin 1 ; Zhang, Jianguo 1 ; Zhang, Jining 2 ;

作者机构： 1.Univ Shanghai Sci & Technol, Sch Med Instrument & Food Engn, Inst Food Sci & Engn, 516 Jungong Rd, Shanghai 200093, Peoples R China

2.Shanghai Acad Agr Sci, Ecoenvironm Protect Res Inst, Shanghai, Peoples R China

关键词： Gene knockout; Komagataella phaffii; mixed carbon source feeding; xylanase

期刊名称：BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT （ 影响因子：1.632； 五年影响因子：2.029 ）

ISSN： 1310-2818

年卷期： 2019 年 33 卷 1 期

页码：

收录情况： SCI

摘要： Xylanase is widely used in agriculture and food industry. To increase heterologous xylanase expression by Komagataella phaffii under the condition of mixed carbon source feeding, the atg30 gene was knocked out from this yeast to inhibit pexophagy for increasing sorbitol consumption. Komagataella phaffii atg30 Delta was constructed through homologous recombination that resulted in the inhibition of pexophagy. When compared with the control, the yield of secretory xylanase increased by 4.1 +/- 0.2%, 5.7 +/- 0.5%, 11.8 +/- 0.4% and 11.4 +/- 0.7% under the conditions of 1.5% methanol and 0.5, 1, 1.5 and 2% sorbitol addition, respectively. The residual sorbitol analysis showed that sorbitol consumed by Komagataella phaffii atg30 Delta increased by 121.3 +/- 9.3%, 66.8 +/- 15.2% and 31.2 +/- 3.2% following 1, 1.5 and 2 sorbitol addition, respectively. Thus, non-methanol carbon source, co-feeding with methanol, provided more energy without pexophagy by atg30 gene knockout. Pexophagy inhibition increased the heterologous protein expression by Komagataella phaffii, which provided basis for increased yield of xylanase through pexophagy monitoring.

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