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Genomic organization and sexually dimorphic expression of the Dmrt1 gene in largemouth bass (Micropterus salmoides)

文献类型： 外文期刊

作者： Yan, Ningning 1 ; Hu, Jie 1 ; Li, Jia 3 ; Dong, Junjian 1 ; Sun, Chengfei 1 ; Ye, Xing 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Inst, Key Lab Trop & Subtrop Fisheries Resource Applica, Minist Agr, Guangzhou 510380, Guangdong, Peoples R China

2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

3.BGI Shenzhen, Guangdong Prov Key Lab Mol Breeding Marine Econ A, Shenzhen Key Lab Marine Genom, Shenzhen 518083, Peoples R China

关键词： Doublesex and Mab-3-related transcription factor 1 gene (Dmrt1); Dimorphic expression; Genomic organization; Micropterus salmoides

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY （ 影响因子：2.231； 五年影响因子：2.215 ）

ISSN： 1096-4959

年卷期： 2019 年 234 卷

页码：

收录情况： SCI

摘要： Doublesex and Mab-3 related transcription factor (Dmrt) genes play important roles in the process of sex determination and differentiation. In this study, a Dmrt1 gene open reading frame sequence was obtained from the gonadal transcriptome data of largemouth bass (Micropterus salmoides), and identified by cloning and sequencing. The ORF of Dmrt1 is 900 bp long, encodes 298 amino acids, and contains the DM region which is characteristic of Dmrt1. Full gDNA sequence of Dmrt1 was composed of five exons and four introns. RT-PCR and Q-PCR analysis of Dmrt1 were conducted in eight tissues and three developmental stages of mature male and female individuals. In situ hybridization was used to locate the expression of Dmrt1 in the testis and ovary of largemouth bass. The results showed that Dmrt1 was highly expressed in the testis of mature fish, but only weakly expressed in other tissues such as heart, liver, and brain, and exhibited gender dimorphism in the gonads of male and female fish at different stages. Furthermore, the expression level in female fish was very low and decreased gradually with ovary maturation. In situ hybridization indicated positive signals were found in early oocytes, but not in mature oocytes, while strong positive signals were found in all types of mature testis cells. The study showed that the sequence and structure of Dmrt1 were highly conserved and exhibited significant gender dimorphism in largemouth bass, as in other fish species. It is suggested that Dmrt1 plays an important role in sex determination and differentiation in largemouth bass.

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