Highly selective molecular imprinting electrochemiluminescence switch sensor for biotoxin L-canavanine measurement
文献类型： 外文期刊
作者： Li, Shuhuai 1 ; Li, Jianping 3 ; Ma, Xionghui 1 ; Liu, Chunhua 1 ; Pang, Chaohai 1 ; Luo, Jinhui 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Anal & Test Ctr, Haikou 571101, Hainan, Peoples R China
2.Hainan Prov Key Lab Qual & Safety Trop Fruits & V, Haikou 571101, Hainan, Peoples R China
3.Guilin Univ Technol, Coll Chem & Bioengn, Guilin 541004, Peoples R China
关键词： Electrochemiluminescence; Switch; Dual-recognition; L-canavanine; Complex
期刊名称：MICROCHEMICAL JOURNAL （ 影响因子：4.821； 五年影响因子：4.364 ）
ISSN： 0026-265X
年卷期： 2019 年 148 卷
页码：
收录情况： SCI
摘要： An electrochemiluminescence (ECL) switch sensor with a dual-recognition mechanism was developed based on complexation reactions and a molecular imprinted film (MIP). The MIP was prepared on a gold electrode with ferroferric oxide/carbon quantum dots (Fe3O4/C-dots) via electrochemical polymerization. An L-canavanine trinitrobenzene complex was used as the template molecule and o-phenylenediamine was used as the functional monomer. Further, C-dots were used as the ECL signal elements and the L-canavanine-trinitrobenzene complex could quench the ECL signal. Subsequently, by using the elution and re-adsorption of the template molecule as the control switch, it was possible to control the ECL intensity of the sensor, and establish a new method for rapidly measuring L-canavanine. A sensor that combined complexation recognition and imprinting recognition demonstrated excellent performance in terms of recognizing specific target :molecules. The detection limits of this method reached 7.32 x 10(-11) mol L-1, and the measurement results for practical samples were satisfactory.
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