Cholesterol-25-hydroxylase Is a Chicken ISG That Restricts ALV-J Infection by Producing 25-hydroxycholesterol
文献类型： 外文期刊
作者： Xie, Tingting 1 ; Feng, Min 1 ; Dai, Manman 3 ; Mo, Guodong 1 ; Ruan, Zhuohao 1 ; Wang, Guiyan 1 ; Shi, Meiqing 4 ; Zhang 1 ;
作者机构： 1.South China Agr Univ, Coll Anim Sci, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Guangdong, Peoples R China
2.Minist Agr, Key Lab Chicken Genet Breeding & Reprod, Guangzhou 510642, Guangdong, Peoples R China
3.South China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China
4.Univ Maryland, Virginia Maryland Reg Coll Vet Med, Div Immunol, College Pk, MD 20742 USA
关键词： chicken; ISG; ALV-J; CH25H
期刊名称：VIRUSES-BASEL （ 影响因子：5.048； 五年影响因子：5.127 ）
ISSN： 1999-4915
年卷期： 2019 年 11 卷 6 期
页码：
收录情况： SCI
摘要： The avian leukosis virus subgroup J (ALV-J) belongs to the chicken retrovirus that causes enormous economic losses in the poultry industry. Interferon-stimulated genes (ISGs) are critical for controlling virus infections. Here, we identified 897 type I ISGs induced by interferon-alpha (IFN-alpha) in chicken peripheral blood mononuclear cell (PBMC) by RNA-Seq. In addition, we further identified 152 potential anti-ALV-J chicken type I ISGs. Among these potential anti-ALV-J ISGs, chicken cholesterol 25-hydroxylase (chCH25H) was selected for further antiviral mechanism studies in chicken embryo fibroblast cell lines (DF1). The gene chCH25H is located on chromosome 6 and clustered in a distinct group with mammals CH25H in the phylogenetic tree. The core promoter region of chCH25H was located within -75/-1 sequence. We found that chCH25H was induced by chicken IFN-alpha and ALV-J in DF1 cells. The overexpression of chCH25H significantly inhibited ALV-J replication in DF1 cells at 48 h post infection (hpi). In addition, ALV-J replication was significantly enhanced in the chCH25H- knockout DF1 cells. Furthermore, we demonstrated that chCH25H restricted ALV-J infection through the production of 25-hydroxycholesterol (25HC), rather than type I and II interferon. Our results identified 152 potential anti-ALV-J chicken type I ISGs and revealed that 25HC, the product of chCH25H, could be used as a natural antiviral agent to control ALV-J infection.
- 相关文献
作者其他论文 更多>>
-
The transmembrane protein TMEM182 promotes fat deposition and alters metabolomics and lipidomics
作者：Chen, Genghua;Lin, Zetong;Peng, Haoqi;Zhang, Shuai;Zhang, Zihao;Zhang, Xiquan;Nie, Qinghua;Luo, Wen;Chen, Genghua;Lin, Zetong;Peng, Haoqi;Zhang, Shuai;Zhang, Zihao;Zhang, Xiquan;Nie, Qinghua;Chen, Genghua;Lin, Zetong;Peng, Haoqi;Zhang, Shuai;Zhang, Zihao;Zhang, Xiquan;Nie, Qinghua
关键词：TMEM182; Fat formation; Metabolomics; Lipidomics
-
Effect of an Ipomoea aquatica Floating Raft on the Water Quality, Antioxidant System, Non-Specific Immune Responses, and Microbial Diversity of Penaeus vannamei in an Aquaculture System
作者：Ruan, Zhuohao;Xie, Ruilin;Li, Yifu;Luo, Yuanyuan;Liu, Wensheng;Ruan, Zhuohao;Ruan, Zhuohao;Li, Yifu;Liu, Wensheng;Weng, Zufeng;Liu, Wensheng
关键词：Pacific white shrimp; water spinach; shrimp-vegetable symbiosis; integrated agri-aquaculture systems; aquaculture
-
PMAIP1 promotes J subgroup avian leukosis virus replication by regulating mitochondrial function
作者：Zhao, Yongxia;Zhao, Changbin;Pan, Ming;Mo, Guodong;Liao, Zhiying;Zhang, Xiquan;Zhang, Dexiang;Li, Hongmei;Zhao, Yongxia;Zhao, Changbin;Deng, Yuelin;Pan, Ming;Mo, Guodong;Liao, Zhiying;Zhang, Xiquan;Zhang, Dexiang;Li, Hongmei;Zhao, Yongxia;Zhao, Changbin;Deng, Yuelin;Pan, Ming;Mo, Guodong;Liao, Zhiying;Zhang, Xiquan;Zhang, Dexiang;Li, Hongmei;Zhao, Yongxia;Zhao, Changbin;Deng, Yuelin;Pan, Ming;Mo, Guodong;Liao, Zhiying;Zhang, Xiquan;Zhang, Dexiang;Li, Hongmei;Deng, Yuelin
关键词：ALV-J; PMAIP1; mitochondrial function; immune; DF-1
-
Live chicken body fat measurement technology based on bio-electrical impedance
作者：Liang, Jinping;Li, Zhenhui;Nie, Qinghua;Zhang, Dexiang;Zhang, Xiquan;Li, Hongmei;Zuo, Jiaming;Cheng, Shangshang;Li, Zhen;Zuo, Jiaming;Cheng, Shangshang;Li, Zhen;Liang, Jinping;Li, Zhenhui;Nie, Qinghua;Zhang, Dexiang;Zhang, Xiquan;Li, Hongmei;Liang, Jinping;Li, Zhenhui;Nie, Qinghua;Zhang, Dexiang;Zhang, Xiquan;Li, Hongmei;Liang, Jinping;Li, Zhenhui;Nie, Qinghua;Zhang, Dexiang;Zhang, Xiquan;Li, Hongmei;Deng, Yuelin
关键词：BIA; Electrical impedance; Fat content; Broilers
-
Whole-transcriptome sequencing revealed the ceRNA regulatory network during the proliferation and differentiation of goose myoblast
作者：Zhang, Xiquan;Luo, Wen;Zhang, Xiquan;Luo, Wen;Xiao, Liangchao;Chen, Jiahui;He, Xueying;Zhang, Xiquan;Luo, Wen;Xiao, Liangchao;Chen, Jiahui;He, Xueying;Zhang, Xiquan;Luo, Wen;Xiao, Liangchao;Chen, Jiahui;He, Xueying;Zhang, Xiquan;Luo, Wen
关键词：Shitou goose; skeletal muscle; whole-transcriptome sequencing; lncRNA; ceRNA network
-
LncEDCH1 g.1703613 T>C regulates chicken carcass traits by targeting miR-196-2-3p
作者：Yuan, Rongshuai;Cai, Bolin;Ma, Manting;Zhao, Changbin;Xian, Yuanrong;Nie, Qinghua;Zhang, Xiquan;Zhang, Dexiang;Zhang, Dexiang
关键词：LncEDCH1; miR-196-2-3p; single nucleotide polymorphism; carcass trait; chicken
-
Weighted single-step GWAS identified candidate genes associated with carcass traits in a Chinese yellow-feathered chicken population
作者：Pan, Rongyang;Qi, Lin;Xu, Zhenqiang;Zhang, Dexiang;Nie, Qinghua;Zhang, Xiquan;Luo, Wen;Pan, Rongyang;Qi, Lin;Xu, Zhenqiang;Zhang, Dexiang;Nie, Qinghua;Zhang, Xiquan;Luo, Wen;Pan, Rongyang;Pan, Rongyang;Qi, Lin;Zhang, Dexiang;Nie, Qinghua;Zhang, Xiquan;Luo, Wen;Pan, Rongyang;Qi, Lin;Xu, Zhenqiang;Zhang, Dexiang;Nie, Qinghua;Zhang, Xiquan;Luo, Wen;Pan, Rongyang;Qi, Lin;Xu, Zhenqiang;Zhang, Dexiang;Nie, Qinghua;Zhang, Xiquan;Luo, Wen
关键词：Chinese yellow-feathered chicken; weighted single-step GWAS; carcass traits; SNP
