文献类型： 外文期刊
作者： Feng, Mingfang 1 ; Cai, Hua 1 ; Guan, Ying 1 ; Sun, Jian 2 ; Zhang, Liguo 3 ; Cang, Jing 1 ;
作者机构： 1.Northeast Agr Univ, Coll Life Sci, Harbin 150030, Heilongjiang, Peoples R China
2.Northeast Agr Univ, Coll Agr, Harbin 150030, Heilongjiang, Peoples R China
3.Heilongjiang Acad Agr Sci, Harbin 150086, Heilongjiang, Peoples R China
关键词： transgenic FGF21 rice; agronomic traits analysis; physiological analysis; SEM; proteome analysis
期刊名称：BREEDING SCIENCE （ 影响因子：2.086； 五年影响因子：2.632 ）
ISSN： 1344-7610
年卷期： 2019 年 69 卷 2 期
页码：
收录情况： SCI
摘要： Although some studies have been conducted on the effects of foreign protein expression on rice, the results vary with foreign gene types and protein expression. This study reveals the effects of fibroblast growth factor 21 (FGF21) expression on mature rice seeds in various aspects. Results revealed that the grain weight of the transgene rice was lower than that of non-transgenic wild-type. The sucrose content and ADP-glucose pyrophosphorylase (AGPase) activity in transgenic FGF21 rice were higher than that in non-transgenic wild-type rice, while changes in the starch content, starch branching enzyme (SBE), sucrose synthase (SuS), superoxide dismutase (SOD) and peroxidase (POD) activity were lower in transgenic FGF21 rice compared to non-transgenic wild-type. The scanning electron microscope results revealed that mature seeds of the transgenic FGF21 rice contained fewer vascular bundles with irregular arrangement compared to the wild-type. The mature seeds of CK and T1 rice lines were collected for proteome analysis, and 167 differentially expressed proteins (DEPs) were found. In addition, the most enriched pathways in both rice lines were determined to be amino sugar and nucleotide sugar metabolism and starch and sucrose metabolism, etc. This study laid the foundation for revealing the effects of exogenous protein expression on rice bioreactors.
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