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The R2R3-MYB transcription factor PaMYB10 is involved in anthocyanin biosynthesis in apricots and determines red blushed skin

文献类型： 外文期刊

作者： Xi, Wanpeng 1 ; Feng, Jing 2 ; Liu, Yu 2 ; Zhang, Shikui 3 ; Zhao, Guohua 1 ;

作者机构： 1.Southwest Univ, Coll Food Sci, Chongqing 400715, Peoples R China

2.Southwest Univ, Coll Hort & Landscape Architecture, Chongqing 400716, Peoples R China

3.Xinjiang Acad Agr Sci, Agr Natl Fruit Tree Germplasm Repository, Luntai 841600, Xinjiang, Peoples R China

关键词： Prunus armeniaca; Anthocyanin; MYB transcription factor; WGCNA; Cloning; qRT-PCR

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.215； 五年影响因子：4.96 ）

ISSN： 1471-2229

年卷期： 2019 年 19 卷

页码：

收录情况： SCI

摘要： BackgroundThe majority of apricot (Prunus armeniaca L.) cultivars display orange or yellow background skin, whereas some cultivars are particularly preferred by consumers because of their red blushed skin on the background.ResultsIn this study, two blushed (Jianali' and Hongyu') and two nonblushed (Baixing' and Luntaixiaobaixing') cultivars were used to investigate the formation mechanism of blushed skin in apricots. High-performance liquid chromatography (HPLC) analysis showed that the blushed cultivars accumulated higher cyanidin-3-O-glucoside, cyanidin-3-O-rutinoside and peonidin-3-O-rutinoside levels during fruit ripening than the nonblushed cultivars. Based on coexpression network analysis (WGCNA), a putative anthocyanin-related R2R3-MYB, PaMYB10, and seven structural genes were identified from transcriptome data. The phylogenetic analysis indicated that PaMYB10 clustered in the anthocyanin-related MYB clade. Sequence alignments revealed that PaMYB10 contained a bHLH-interaction motif ([DE]Lx2[RK]x3Lx6Lx3R) and an ANDV motif. Subcellular localization analysis showed that PaMYB10 was a nuclear protein. Real-time qRT-PCR analysis demonstrated that the transcript levels of PaMYB10 and seven genes responsible for anthocyanin synthesis were significantly higher in blushed than in nonblushed apricots, which was consistent with the accumulation of anthocyanin. In addition, bagging significantly inhibited the transcript levels of PaMYB10 and the structural genes in Jianali' and blocked the red coloration and anthocyanin accumulation. Transient PaMYB10 overexpression in Luntaixiaobaixing' fruits resulted in the red blushed skin at the maturation stage.ConclusionsTaken together, these data reveal that three anthocyanins are responsible for the blushed skin of apricots, identify PaMYB10 as a positive regulator of anthocyanin biosynthesis in apricots, and demonstrate that blush formation depends on light.

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