文献类型： 外文期刊

作者： Wang, Haihai ¹ ; Wang, Xiaoqing ⁶ ; Song, Weimeng ¹ ; Bao, Yan ² ; Jin, Yanli ¹ ; Jiang, Chunmei ⁵ ; Wang, Cuiting ¹ ; Li ¹ ;

作者机构： 1.Chinese Acad Sci, Shanghai Inst Plant Physiol & Ecol, Natl Key Lab Plant Mol Genet, 300 Fenglin Rd, Shanghai, Peoples R China

2.Ludong Univ, Coll Agr, 186 Hongqizhong Rd, Yantai, Peoples R China

3.Ludong Univ, Inst Adv Study Coastal Ecol, 186 Hongqizhong Rd, Yantai 264025, Peoples R China

4.Ludong Univ, Key Lab Mol Module Based Breeding High Yield & Ab, 186 Hongqizhong Rd, Yantai 264025, Peoples R China

5.Hubei Univ, Coll Life Sci, Hubei Collaborat Innovat Ctr Green Transformat Bi, 368 Youyi Ave, Wuhan, Hubei, Peoples R China

6.Shanghai Acad Agr Sci, Forestry & Pomol Res Inst, 1000 Jinqi Rd, Shanghai, Peoples R China

关键词： Anthocyanins; PdMYB118; Poplar; Red leaf; Transcription factor

期刊名称：PLANT CELL REPORTS （ 影响因子：4.57； 五年影响因子：4.463 ）

ISSN： 0721-7714

年卷期： 2019 年 38 卷 8 期

页码：

收录情况： SCI

摘要： Key messageA new anthocyanin biosynthesis transcription factor PdMYB118, which could be used for the genetic engineering of colorful tree species, was indentified from a red leaf mutant of Populus deltoids.AbstractIn higher plants, the biosynthesis of anthocyanins is regulated by several classes of transcription factors (TFs), including R2R3-MYB, bHLH and WD-repeat proteins. In this work, we isolated an MYB gene regulating anthocyanin biosynthesis from a red leaf mutant of Populus deltoids, which accumulated more anthocyanins in the leaves and showed higher expression levels of anthocyanin biosynthesis genes than did the wild type. Gene expression analyses of all TFs regulating anthocyanin biosynthesis demonstrated that only a MYB118 homologous gene, PdMYB118, was up-regulated in the mutant compared with the wide type. Subcellular localization analyses in poplar leaf mesophyll protoplasts showed that PdMYB118-YFP fusion protein was specifically located in nucleus. When transiently expressed in poplar leaf protoplasts, PdMYB118 specifically promoted the expression of anthocyanidin biosynthesis genes. Dual-luciferase assays revealed that PdMYB118 can directly activate the promoters of these genes. When overexpressed in Shanxin Yang (P. davidianaxP. bolleana), a hybrid clone commercially grown for landscaping in the northern part of China, transgenic plants overexpressing PdMYB118 produced more anthocyanins in the leaves and turned their color into redness when grown in both greenhouse and field. Consistently, transcripts of some important anthocyanidin biosynthesis genes were significantly increased in the leaves of transgenic plants. All these results indicate that PdMYB118 functions as an essential transcription factor regulating anthocyanin biosynthesis in poplar and could be used for the genetic engineering of colorful tree species.