Development of an oligonucleotide dye solution facilitates high throughput and cost-efficient chromosome identification in peanut
文献类型： 外文期刊
作者： Du, Pei 1 ; Cui, Caihong 1 ; Liu, Hua 1 ; Fu, Liuyang 3 ; Li, Lina 1 ; Dai, Xiaodong 1 ; Qin, Li 1 ; Wang, Siyu 3 ; Han, Suo 1 ;
作者机构： 1.Henan Acad Agr Sci, Henan Prov Key Lab Oil Crops Improvement, Key Lab Oil Crops Huang Huai Hai Plains, Minist Agr,Ind Crops Res Inst, Zhengzhou 450002, Henan, Peoples R China
2.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China
3.Zhengzhou Univ, Sch Life Sci, Zhengzhou 450001, Henan, Peoples R China
关键词： Arachis species; Oligonucleotide dye solution; FISH; Karyotype
期刊名称：PLANT METHODS （ 影响因子：4.993； 五年影响因子：5.312 ）
ISSN： 1746-4811
年卷期： 2019 年 15 卷
页码：
收录情况： SCI
摘要： BackgroundDevelopment of oligonucleotide probes facilitates chromosome identification via fluorescence in situ hybridization (FISH) in many organisms.ResultsWe report a high throughput and economical method of chromosome identification based on the development of a dye solution containing 2xsaline-sodium citrate (SSC) and oligonucleotide probes. Based on the concentration, staining time, and sequence effects of oligonucleotides, an efficient probe dye of peanut was developed for chromosome identification. To validate the effects of this solution, 200 slides derived from 21 accessions of the cultivated peanut and 30 wild Arachis species were painted to identify Arachis genomes and establish karyotypes. The results showed that one jar of dye could be used to paint 10 chromosome preparations and recycled at least 10 times to efficiently dye more than 100 slides. The A, B, K, F, E, and H genomes showed unique staining karyotype patterns and signal colors.ConclusionsBased on the karyotype patterns of Arachis genomes, we revealed the relationships among the A, B, K, F, E, and H genomes in genus Arachis, and demonstrated the potential for adoption of this oligonucleotide dye solution in practice.
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