Phage-displayed nanobody based double antibody sandwich chemiluminescent immunoassay for the detection of Cry2A toxin in cereals
文献类型： 外文期刊
作者： Qiu, Yulou 1 ; Li, Pan 1 ; Liu, Beibei 1 ; Liu, Yuan 1 ; Wang, Yulong 1 ; Tao, Tingting 1 ; Xu, Junli 1 ; Hammock, Bruce 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Key Lab Control Technol & Stand Agroprod Safety &, State Key Lab Cultivat Base,Minist Sci & Technol, Minist Agr,Inst Food Safety & Nutr,Lab Food Qual, Nanjing 210014, Jiangsu, Peoples R China
2.China Jiliang Univ, Zhejiang Proceincial Key Lab Biometrol & Inspect, Hangzhou 310018, Zhejiang, Peoples R China
3.Univ Calif Davis, Dept Entomol, Lab Pesticide & Nematol Biotechnol, Davis, CA 95616 USA
4.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Jiangsu, Peoples R China
关键词： Cry2A toxin; phage-displayed nanobody; chemiluminescence; sandwich immunoassay
期刊名称：FOOD AND AGRICULTURAL IMMUNOLOGY （ 影响因子：3.101； 五年影响因子：2.573 ）
ISSN： 0954-0105
年卷期： 2019 年 30 卷 1 期
页码：
收录情况： SCI
摘要： In this study, we developed a double antibody sandwich chemiluminescent immunoassay (DAS-CLIA) based on phage-displayed nanobodies for the determination of Cry2A toxin. Nine phage-displayed nanobodies specifically to Cry2A were obtained from a naive nanobody library after four rounds of biopanning. The phage-displayed nanobody P2 with high affinity binding was selected as the detection antibody for the sensitive DAS-CLIA development. The proposed method exhibited high sensitivity with a limit of detection of 0.09 ng/mL, has a wide linear range for Cry2A toxin detection, in the range of 0.1-1000 ng/mL and negligible cross-reactivities to the other Bt toxins. The recoveries of Cry2A toxin spiked in cereal samples (rice, wheat, and corn) ranged from 82.7% to 118%, with a coefficient of variation of less than 10%. This study demonstrated that the DAS-CLIA procedure based on phage-displayed nanobodies may provide an alternative sensitive method for the detection of Bt toxins in agri-products. [GRAPHICS] .
- 相关文献
作者其他论文 更多>>
-
Combined detection of foodborne pathogens in irrigation water in Jiangsu, China
作者：Xie, Yajing;Zhang, Cunzheng;Liu, Xianjin;Xie, Yajing;Lu, Jiayao;Liu, Xianjin;Li, Bin;Fan, Bochao;Li, Dandi;Du, Xuefei;Xie, Yajing;Schmidt, Stefan
关键词：Irrigation water; Foodborne pathogens; Combined detection; Noroviruses; Strawberry production
-
A fast and efficient virtual screening and identification strategy for helix peptide binders based on finDr webserver: A case study of bovine serum albumin (BSA)
作者：Bu, Jiarui;Luo, Na;Liu, Yuan;Luo, Chuping;Zhang, Xiao;Bu, Jiarui;Luo, Na;Shen, Cheng;Xu, Chongxin;Zhu, Qing;Chen, Chengyu;Xie, Yajing;Liu, Xianjin;Liu, Yuan;Zhang, Xiao
关键词：Virtual screening; Affinity identification; Molecular simulations
-
Using designed polypeptides to develop novel phage-mediated triple antibody sandwich immunoassay for the specific detection of Escherichia coli O157:H7 in milk
作者：Xue, Chang;Ouyang, Qin;Zhang, Cunzheng;Xue, Chang;Fu, Jinmei;Tao, Tingting;Zhu, Shouhu;Han, Bingsong;Wang, Yulong;Ye, Yuhui;Zhang, Cunzheng;Fu, Jinmei;Zhang, Cunzheng;Zhu, Shouhu;Zhang, Cunzheng;Hammock, Bruce D.;Hammock, Bruce D.;Luo, Ruifeng
关键词：Polypeptides; Phage-displayed nanobody; Serotype discrimination; Triple antibody sandwich immunoassay; Escherichia coli O157:H7
-
An anti-idiotypic single domain antibody as Cry2Aa toxin mimotope and analysis of its binding region through molecular docking
作者：Lin, Manman;Han, Guangjie;Li, Chuanming;Huang, Lixin;Liu, Qin;Zhang, Nan;Xia, Yang;Lu, Yurong;Xu, Jian;Liu, Xianjin;Liu, Yuan;Lin, Johnson
关键词：Cry2Aa toxin; Anti-idiotypic antibody; Single-domain antibody; Epitopes; Mimotope; Molecular docking
-
Development of colorimetric immunochromatographic assay based on different morphologies of gold nanoparticles for the detection of triadimefon
作者：Dong, Sa;Zhang, Yu;Shi, Qiuyun;Zhu, Zixin;Feng, Jianguo;Wang, Yulong;Luo, Ruifeng
关键词：Triadimefon; Immunochromatographic strip; Gold nanoparticles; Gold nanoflowers; Gold nanorods; Monoclonal antibody
-
A Novel Camel Milk-Derived Peptide LLPK Improves Glucose-Lipid Metabolism in db/db Mice via PPAR Signaling Pathway
作者：Han, Binsong;Ye, Yuhui;Zhang, Cunzheng;Han, Binsong;Zhang, Lina;Zhou, Peng;Han, Binsong;Zhang, Lina;Zhou, Peng
关键词：camel milk-derived peptide; diabetes; hepatic proteome; PPAR signaling pathway
-
Minimalist nanobody architecture: A double-edged sword for immunological analysis
作者：Wang, Yulong;Liu, Pengyan;Zhang, Yaodong;Zhang, Cunzheng;Wang, Yulong;Luo, Ruifeng;Yang, Huijuan;Dong, Sa;Hua, Xiude;Hammock, Bruce D.
关键词：Nanobody-based immunoassay; Miniaturized structure; Small size; Bioconjugation chemistry
