Cloning, functional analysis, and microRNA-induced negative regulation of growth arrest and DNA damage-inducible 45 gamma (Gadd45g) in grass carp (Ctenopharyngodon idella)
文献类型： 外文期刊
作者： Fang, Yuan 1 ; Xu, Xiao-Yan 1 ; Zhang, Meng 1 ; Bai, Yulin 1 ; Zhang, Xue-shu 1 ; Shen, Yubang 1 ; Li, Jiale 1 ;
作者机构： 1.Shanghai Ocean Univ, Minist Agr, Key Lab Freshwater Aquat Genet Resources, Shanghai, Peoples R China
2.Chinese Acad Fishery Sci, Key Lab Freshwater Fisheries & Gennplasm Resource, Wuxi 214081, Jiangsu, Peoples R China
3.Shanghai Ocean Univ, Shanghai Engn Res Ctr Aquaculture, Shanghai, Peoples R China
关键词： Grass carp; Ctenopharyngodon idella; CiGadd45g; Aeromonas hydrophila; microRNA; Innate immunity
期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.636； 五年影响因子：3.654 ）
ISSN： 0145-305X
年卷期： 2019 年 99 卷
页码：
收录情况： SCI
摘要： Growth arrest and DNA damage-inducible 45 gamma (Gadd45g) is a member of Gadd45 gene family of immunological proteins in mammals. Herein, we identified and characterised Gadd45g from grass carp. The cDNA spans over 1189 bp, with an open reading frame of 480 bp encoding a 159 amino acid protein. CiGadd45g mRNAs were expressed in all tissues investigated, with abundant expression in liver, kidney, heart, brain, blood and skin. Following infection with Aeromonas hydrophila, CiGadd45g expression was upregulated in these immune-related tissues (gill, liver, spleen, intestine, kidney and head kidney). Immune-related cytokines (p38 and JNK) and proinflammatory cytokines (IL-8, IFN-1 and TNF-alpha) were activated by CiGadd45g. CiGadd45g and downstream genes were regulated by microRNA miR-429b. These results indicate that CiGadd45g plays an important immune role in the response to A. hydrophila infection in grass carp.
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