文献类型： 外文期刊
作者： Cheng, Xi 1 ; Huang, Conglin 2 ; Zhang, Xiuhai 2 ; Lyu, Yingmin 1 ;
作者机构： 1.Beijing Forestry Univ, Beijing Key Lab Ornamental Germplasm Innovat & Mo, China Natl Engn Res Ctr Floriculture, Coll Landscape Architecture, Beijing 100083, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing 100097, Peoples R China
关键词： Marigold; Tagetes erecta; GFP gene; Floral dip; Transforming system
期刊名称：ACTA PHYSIOLOGIAE PLANTARUM （ 影响因子：2.354； 五年影响因子：2.711 ）
ISSN： 0137-5881
年卷期： 2019 年 41 卷 8 期
页码：
收录情况： SCI
摘要： The floral dip transformation method avoids tissue culture and regeneration processes of marigold, and the green fluorescent protein (GFP) is widely used in the detection of genetically modified organisms. In our study, the binary vector PCB260 containing the screening gene basta, the reporter gene GFP, and a 4x35S enhancer was transferred into marigold (Tagetes erecta) via Agrobacterium tumefaciens EHA105-mediated transformation using the floral dip method. After herbicide-resistance screening and genomic PCR testing, four transgenic lines were obtained in T-0 generation. In the T-1 generation, 15 transgenic plants showed fluorescence and were GFP-positive with phenotypic changes. The segregation ratio of mutant to normal plants was 1:3. Plant height, leaf length and width were significantly greater in the normal plants than in the mutant plants. Mutant plants did not bloom.
- 相关文献
作者其他论文 更多>>
-
Auxin regulates bulbil development by affecting carbohydrate metabolism in Lilium lancifolium
作者：Wang, Mengdi;Li, Jiamin;Zhang, Xuemin;Kang, Shengnan;Yu, Xiaonan;Wang, Mengdi;Li, Jiamin;Pan, Wenqiang;Sang, Qianzi;Liang, Jiahui;Zhang, Xuemin;Kang, Shengnan;Zhang, Mingfang;Zhang, Xiuhai;Du, Yunpeng;Wang, Mengdi;Li, Jiamin;Pan, Wenqiang;Sang, Qianzi;Liang, Jiahui;Zhang, Xuemin;Kang, Shengnan;Zhang, Mingfang;Zhang, Xiuhai;Du, Yunpeng;Sang, Qianzi
关键词：Lilium lancifolium; Bulbil development; Transcriptome; Auxin; Carbohydrate metabolism
-
Salicylic acid influences the resistance of colored calla lily to soft rot (Pectobacterium carotovorum) via the TGA2-LNC88940-miR528-SOD module
作者：Wang, Yi;Wang, Di;Gou, Rongxin;Jiang, Yin;Zeng, Zhen;Zhang, Xiuhai;Wei, Zunzheng;Yang, Tuo;Wang, Di;Gou, Rongxin;Jiang, Yin;Zhang, Guojun
关键词：soft rot;
Zantedeschia elliottiana ; lncRNA; miRNA; salicylic acid -
The giant genome of lily provides insights into the hybridization of cultivated lilies
作者：Liang, Yuwei;Gao, Qiang;Zhang, Liangsheng;Li, Fan;Duan, Qing;Li, Shenchong;Jin, Chunlian;Zhang, Peihua;Wang, Jihua;Li, Fan;Duan, Qing;Li, Shenchong;Jin, Chunlian;Zhang, Peihua;Wang, Jihua;Du, Yunpeng;Pan, Wenqiang;Zhang, Xiuhai;Zhang, Mingfang;Wu, Jian;Pan, Wenqiang;Wang, Shaokun;Song, Xiaoming;Zhong, Linlin;Zhang, Fan;Li, Yan;Wang, Zhiwei;Li, Danqing;Gu, Yang;Chen, Zhong-Hua;Chen, Zhong-Hua;Mayer, Klaus F. X.;Mayer, Klaus F. X.;Zhou, Xiaofan;Zhang, Liangsheng;Zhang, Liangsheng
关键词：
-
Molecular Elucidation of Anthocyanin Accumulation Mechanisms in Hippeastrum hybridum Cultivars
作者：Guo, Pengyu;Xing, Chuanji;Ye, Jiacheng;Xue, Jing;Zhang, Xiuhai;Chen, Xuqing;Guo, Pengyu;Hu, Zongli;Chen, Guoping;Ye, Jiacheng;Mur, Luis A. J.;Di, Bao
关键词：
Hippeastrum ; petals; anthocyanins; transcriptomics; metabolomics; WGCNA; Y2H -
The NAC transcription factor LpNAC48 promotes trichome formation in Lilium pumilum
作者：Xin, Yin;Pan, Wenqiang;Wu, Jingxiang;Zhang, Mingfang;Ma, Yang;Zhang, Xiuhai;Du, Yunpeng;Xin, Yin;Pan, Wenqiang;Zhao, Yajie;Li, Jingru;Wang, Shaokun;Wu, Jingxiang;Shi, Jinxin;Ma, Yang;Wu, Jian;Pan, Wenqiang;Zhang, Xiuhai;Du, Yunpeng;Yang, Chenglong;Fang, Shaozhong;Liang, Yuwei;Zaccai, Michele
关键词：
-
A natural variation of flavone synthase II gene enhances flavone accumulation and confers drought adaptation in chrysanthemum
作者：Luo, Jiayi;Han, Mingzheng;Song, Zhenzhen;Zhang, Haixia;Zhao, Yafei;Ma, Chao;Luo, Chang;Huang, Conglin;Wang, Qinrui;Gao, Qiang;Lin, Tao
关键词：Chrysanthemum; drought; flavone; FNSII; GWAS
-
China's chrysanthemum in the global market: evaluating the international competitiveness and influencing factors
作者：Pan, Bo;Chen, Qingbing;Wang, Yaoyao;Chen, Lijie;Huang, Conglin;Gao, Kang;Pan, Bo;Chen, Qingbing;Chen, Lijie;Li, Haiying;Du, Yingxin
关键词：cut chrysanthemums; international competitiveness; bilateral trade; CMS model; trade gravity model
