文献类型： 外文期刊
作者： Cheng, Xi 1 ; Huang, Conglin 2 ; Zhang, Xiuhai 2 ; Lyu, Yingmin 1 ;
作者机构： 1.Beijing Forestry Univ, Beijing Key Lab Ornamental Germplasm Innovat & Mo, China Natl Engn Res Ctr Floriculture, Coll Landscape Architecture, Beijing 100083, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing 100097, Peoples R China
关键词： Marigold; Tagetes erecta; GFP gene; Floral dip; Transforming system
期刊名称：ACTA PHYSIOLOGIAE PLANTARUM （ 影响因子：2.354； 五年影响因子：2.711 ）
ISSN： 0137-5881
年卷期： 2019 年 41 卷 8 期
页码：
收录情况： SCI
摘要： The floral dip transformation method avoids tissue culture and regeneration processes of marigold, and the green fluorescent protein (GFP) is widely used in the detection of genetically modified organisms. In our study, the binary vector PCB260 containing the screening gene basta, the reporter gene GFP, and a 4x35S enhancer was transferred into marigold (Tagetes erecta) via Agrobacterium tumefaciens EHA105-mediated transformation using the floral dip method. After herbicide-resistance screening and genomic PCR testing, four transgenic lines were obtained in T-0 generation. In the T-1 generation, 15 transgenic plants showed fluorescence and were GFP-positive with phenotypic changes. The segregation ratio of mutant to normal plants was 1:3. Plant height, leaf length and width were significantly greater in the normal plants than in the mutant plants. Mutant plants did not bloom.
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