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Screening and validation of optimal miRNA reference genes in different developing stages and tissues of Lilium henryi Baker

文献类型： 外文期刊

作者： Jin, Ge 1 ; Zhang, Xiuhai 1 ; Yu, Shiyin 1 ; Du, Yunpeng 1 ; Wang, Meixian 3 ; Zhao, Chunli 2 ; Zhang, Mingfang 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Key Lab Urban Agr North China, Minist Agr & Rural Affairs, Beijing 100097, Peoples R China

2.Jilin Agr Univ, Coll Forestry & Grassland Sci, Changchun 130118, Peoples R China

3.Beijing Forestry Univ, Coll Landscape Achitecture, Beijing 100097, Peoples R China

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.6； 五年影响因子：4.9 ）

ISSN： 2045-2322

年卷期： 2024 年 14 卷 1 期

页码：

收录情况： SCI

摘要： Dynamic miRNA detection using the qRT-PCR technique requires appropriate reference genes to ensure data reliability. Previous studies have screened internal reference genes in plants during embryonic development and various stress treatment, involving relatively few tissues and organs. There is no relevant miRNA study in Lilium henryi Baker and limited research on the optimal miRNA reference genes in lilies, such as 5S, 18S, U6 and Actin. Twelve genes were selected as candidate reference genes whose expression stability was analyzed in petals at different developmental stages and other tissues using various algorithms, such as geNorm, NormFinder, BestKeeper, and Delta CT. The results revealed that the optimal combination of reference genes for Lilium henryi Baker petals at different developmental stages was osa-miR166m and osa-miR166a-3p, while that for different tissues of Lilium henryi Baker was osa-miR166g-3p and osa-miR166a-3p.Four important genes related to growth and development regulation, namely, osa-miR156a, osa-miR395b, osa-miR396a-3p, and osa-miR396a-5p, were selected for validation. The findings of the present study could contribute to future investigations onmiRNA expression and the related functions in Lilium henryi Baker while providing important references for the normalization of the miRNA expression in other varieties of lily.

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