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Mutation of cyp19a1b results in sterile males due to efferent duct obstruction in Nile tilapia

文献类型： 外文期刊

作者： Zhang, Xianbo 1 ; Min, Qianwen 1 ; Li, Mengru 1 ; Liu, Xingyong 1 ; Li, Minghui 1 ; Wang, Deshou 1 ;

作者机构： 1.Southwest Univ, Sch Life Sci, Key Lab Aquat Sci Chongqing, Key Lab Freshwater Fish Reprod & Dev,Minist Educ, Chongqing, Peoples R China

2.Guizhou Acad Agr Sci, Guizhou Fisheries Res Inst, Guiyang, Guizhou, Peoples R China

关键词： Cyp19a1b; efferent duct obstruction; estrogen; Nile tilapia; sterility

期刊名称：MOLECULAR REPRODUCTION AND DEVELOPMENT （ 影响因子：2.609； 五年影响因子：3.47 ）

ISSN： 1040-452X

年卷期：

页码：

收录情况： SCI

摘要： The involvement of estrogen in male fertility has been well established in mammals. However, less is known about the role of estrogen in fish male reproduction. Our recent study revealed that Cyp19a1a deficiency had no effect on fertility in male fish. In this study, expression of Cyp19a1b, but not Cyp19a1a, was detected by immunohistochemistry in Leydig cells of tilapia testes. cyp19a1b mutation resulted in a significant decrease in the concentration of 17 beta-estradiol in serum and sterility in XY fish, as no offspring were obtained when crossed with control XX fish at 240 days after hatching (dah). No sperm was obtained from the mature mutants by in vitro extrusion. Further examination of the mutant gonads revealed excessive semen accumulation and testicular hypertrophy. Semen collected from the mutant testes during autopsy contained sperm with a normal morphology that showed no significant differences in motility, VCL, BCF, STR, or fertility compared with control sperm. Efferent ducts from the mutant testes, which had low-convolution levels, fewer branches, and no blood vessels observed inside the walls, were significantly smaller in size. qRT-PCR analyses showed downregulated expression of ion exchange genes. There was increased apoptosis in the epithelial cells of the efferent ducts and other somatic cells of the testes as revealed by TUNEL staining, as well as upregulation of apoptosis gene expression in the mutants. At 360 dah, mutant fish showed testicular atrophy and efferent duct fibrosis. These results demonstrated that estrogen deficiency caused by Cyp19a1b mutation resulted in male sterility due to efferent duct obstruction.

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