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The revised complete mitogenome sequence of the tree frog Polypedates megacephalus (Anura, Rhacophoridae) by next-generation sequencing and phylogenetic analysis

文献类型： 外文期刊

作者： Huang, An 1 ; Liu, Shuo 3 ; Li, Haijun 1 ; Luo, Hongdi 1 ; Ni, Qingyong 1 ; Yao, Yongfang 4 ; Xu, Huailiang 4 ; Zeng, Bo; 1 ;

作者机构： 1.Sichuan Agr Univ, Coll Anim Sci & Technol, Chengdu, Sichuan, Peoples R China

2.Sichuan Agr Univ, Farm Anim Genet Resources Explorat & Innovat Key, Chengdu, Sichuan, Peoples R China

3.Chinese Acad Sci, Kunming Nat Hist Museum Zool, Kunming Inst Zool, Kunming, Yunnan, Peoples R China

4.Sichuan Agr Univ, Coll Life Sci, Yaan, Peoples R China

5.Hebei Acad Agr & Forestry Sci, Inst Millet Crops, Shijiazhuang, Hebei, Peoples R China

关键词： Mitochondrial gene rearrangement; ND5; Phylogenetic relationships; Polypedates megacephalus

期刊名称：PEERJ （ 影响因子：2.984； 五年影响因子：3.369 ）

ISSN： 2167-8359

年卷期： 2019 年 7 卷

页码：

收录情况： SCI

摘要： The mitochondrial genome (mitogenome) sequence of the tree frog Polypedates megacephalus (16,473 bp) was previously reported as having the unusual characteristic of lacking the ND5 gene. In this study, a new mitogenome of P. megacephalus (19,952 bp) was resequenced using the next-generation sequencing (NGS) and standard Sanger sequencing technologies. It was discovered that the ND5 gene was not lost but translocated to the control region (CR) from its canonical location between the ND4 and ND6 genes. In addition, a duplicated control region was found in the new mitogenome of this species. Conservative region identification of the ND5 gene and phylogenetic analysis confirmed that the ND5 gene was located between two control regions. The phylogenetic relationship among 20 related species of anura revealed a rearrangement of the ND5 gene during the evolutionary process. These results also highlighted the advantages of next-generation sequencing. It will not only decrease the time and cost of sequencing, but also will eliminate the errors in published mitogenome databases.

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