文献类型： 外文期刊

作者： Wu, Xiao ¹ ; Shi, Xinjie ¹ ; Bai, Mudan ² ; Chen, Yangyang ¹ ; Li, Xiaolong ¹ ; Qi, Kaijie ¹ ; Cao, Peng ¹ ; Li, Mingzhi; ¹ ;

作者机构： 1.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Ctr Pear Engn Technol Res, Nanjing 210095, Jiangsu, Peoples R China

2.Shanxi Acad Agr Sci, Pomol Res Inst, Jinzhong 030815, Shanxi, Peoples R China

3.Genepioneer Biotechnol Co Ltd, Nanjing 210014, Jiangsu, Peoples R China

关键词： cuticular wax; pear fruit; metabolome; transcriptome; morphology

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN： 0021-8561

年卷期： 2019 年 67 卷 30 期

页码：

收录情况： SCI

摘要： The layer of cuticular wax covering fruits plays important roles in protecting against disease, preventing nonstomatal water loss, and extending shelf life. However, the molecular basis of cuticular wax biosynthesis in pear (Pyrus) fruits remains elusive. Our study thoroughly investigates cuticular wax biosynthesis during pear fruit development from morphologic, transcriptomic, and gas chromatography-mass spectrometry metabolomic perspectives. Our results showed that cuticular wax concentrations increased during the early stage [20-80 days after full bloom (DAFB)] from 0.64 mg/cm(2) (50 DAFB) to 1.75 mg/cm(2) (80 DAFB) and then slightly decreased to 1.22 mg/cm(2) during the fruit ripening period (80-140 DAFB). Scanning electron microscopy imaging indicated that wax plate crystals increased and wax structures varied during the pear fruit development. The combined transcriptomic and metabolomic profiling analysis revealed 27 genes, including 12 genes encoding transcription factors and a new structural gene (Pbr028523) encoding beta-amyrin synthase, participating in the biosynthesis, transport, and regulation of cuticular wax according to their expression patterns in pear fruit. The quantitative real-time polymerase chain reaction experiments of 18 differentially expressed genes were performed and confirmed the accuracy of the RNA-Seq-derived transcript expression. A model of VLCFAs and cuticular wax synthesis and transport in pear fruit is proposed, providing a mechanistic framework for understanding cuticular wax biosynthesis in pear fruit. These results and data sets provide a foundation for the molecular events related to cuticular wax in 'Yuluxiang' pear fruit and may also help guide the functional analyses of candidate genes important for improving the cuticular wax of pear fruit in the future.