Recovery of Muscovy duck-origin goose parvovirus from an infectious clone containing an E-box motif (CACATG) deletion within the left terminal region
文献类型： 外文期刊
作者： Wang, Shao 1 ; Xiao, Shifeng 1 ; Cheng, Xiaoxia 1 ; Chen, Shaoying 1 ; Zhu, Xiaoli 1 ; Lin, Fengqiang 1 ; Chen, Shilon 1 ;
作者机构： 1.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fuzhou 350013, Fujian, Peoples R China
2.Fujian Anim Dis Control Technol Dev Ctr, Fuzhou 350013, Fujian, Peoples R China
关键词： Muscovy duck; Goose parvovirus; E-box motif; Infectious clone
期刊名称：MOLECULAR AND CELLULAR PROBES （ 影响因子：2.365； 五年影响因子：2.386 ）
ISSN： 0890-8508
年卷期： 2019 年 46 卷
页码：
收录情况： SCI
摘要： Muscovy duck-origin goose parvovirus (MDGPV) is a causative agent of MDGPV-associated Derzsy's disease. To evalute the role of the cis-acting element E-box (CACATG) deletion on MDGPV eplication, an infectious plasmid clone p-PT Delta E-287, having one E-box deletion at nucleotide (nt) 287 of the left inverted terminal repeat sequence (L-ITR), was constructed by overlap extension PCR deleting the (287)CACATG(292) motif from the plasmid pMDGPVPT containing the full-length genome of the virulent MDGPV strain PT. The p-PT Delta E-287 plasmid was transfected into 9-day-old non-immune Muscovy duck embryos via the yolk sac, resulting in successful rescue of the deletion mutant virus r-PT Delta E-287. Compared with its parental virus PT, the virulence and the replication ability of r-PM Delta E-287 were reduced. In addition, we examined the ability of r-PT Delta E287 to manipulate cell cycle progression. The results showed that r-PM Delta E-287 replication results in G0/G1 phase accumulation of infected duck embryo liver mesenchymal stem cells (BMSCs) and that this accumulation is caused by the prevention of cell cycle entry from G0/G1 phase into S phase. Taken together, introducing (287)CACATG(292) element deletion into MDGPV PT genomic DNA that induced rescued mutant virus (r-PT Delta(287)) cell cycle arrest function at the G0/G1 phase, which might inhibit MDGPV replication and virus progeny production. This study laid the foundation for further understanding of the relationship between E-box deletion in the L-ITR and MDGPV virulence.
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