文献类型： 外文期刊
作者： Chen, Xiuqin 1 ; Zheng, Min 1 ; Lin, Su 1 ; Huang, Meiqing 1 ; Chen, Shaoying 1 ; Chen, Shilong 1 ;
作者机构： 1.Inst Anim Husb & Vet Med, Fujian Acad Agr Sci, Fuzhou 350013, Fujian, Peoples R China
2.Fujian Anim Dis Control Technol Dev Ctr, Fuzhou 350013, Fujian, Peoples R China
关键词： CRISPR/Cas9; Genome-wide screening; Tumor; Viral infections; Drug resistance
期刊名称：MOLECULAR AND CELLULAR PROBES （ 影响因子：3.0； 五年影响因子：2.6 ）
ISSN： 0890-8508
年卷期： 2025 年 79 卷
页码：
收录情况： SCI
摘要： High-throughput genetic screening serves as an indispensable approach for deciphering gene functions and the intricate relationships between phenotypes and genotypes. The CRISPR/Cas9 system, with its ability to precisely edit genomes on a large scale, has revolutionized the field by enabling the construction of comprehensive genomic libraries. This technology has become a cornerstone for genome-wide screenings in disease research. This review offers a comprehensive examination of how CRISPR/Cas9-based genetic screening has been leveraged to uncover genes that play a role in disease mechanisms, focusing on areas such as cancer development and viral replication processes. The insights presented in this review hold promise for the development of novel therapeutic strategies and precision medicine approaches.
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