Quantum dot bead-based immunochromatographic assay for the quantitative detection of triazophos
文献类型： 外文期刊
作者： Wu, Qin 1 ; Wu, Peiman 3 ; Duan, Hong 1 ; Liu, Beibei 2 ; Shao, Yanna 1 ; Li, Pan 2 ; Zhang, Cunzheng 2 ; Xiong, Yonghua 1 ;
作者机构： 1.Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Jiangxi, Peoples R China
2.Jiangsu Acad Agr Sci, Key Lab Food Qual & Safety Jiangsu Prov, Key Lab Control Technol & Standard Agroprod Safet, State Key Lab Breeding Base,Minist Agr,Inst Food, Nanjing 210014, Jiangsu, Peoples R China
3.Xuzhou Med Univ, Affiliated Hosp, Xuzhou, Jiangsu, Peoples R China
4.Jiangshu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Jiangsu, Peoples R China
关键词： Quantum dot beads; immunochromatographic assay; triazophos; quantitative detection
期刊名称：FOOD AND AGRICULTURAL IMMUNOLOGY （ 影响因子：3.101； 五年影响因子：2.573 ）
ISSN： 0954-0105
年卷期： 2019 年 30 卷 1 期
页码：
收录情况： SCI
摘要： In this study, a quantum dot (QD) bead -based immunochromatographic assay (QB-ICA) was developed for the rapid, sensitive, and quantitative detection of triazophos. QBs with about 640 times brighter luminescence than the corresponding QDs were used as signal-amplified ICA probes. Under optimum conditions, QB-ICA showed a good dynamic linear detection for triazophos at 0.49-250 ng/mL with a half-maximal inhibitory concentration of 6.97 ng/mL. The average recoveries of intra- and inter assays for triazophos-spiked rice samples ranged from 80.91% to 107.00% with the coefficients of variation below 10%, indicating that QB-ICA had an acceptable accuracy for triazophos detection. The triazophos spiked samples were also detected through gas chromatography, and no significant difference (P > 0.05) was found between the two methods. In summary, the proposed QB-ICA system provided a novel platform for the rapid, sensitive, and quantitative detection of triazophos or other pesticides in food materials.
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