A CRISPR/Cas9-Based Study of CgloRPCYG, a Gene That Regulates Pathogenicity, Conidial Yield, and Germination in Colletotrichum gloeosporioides
文献类型： 外文期刊
作者： Zhang, He 1 ; Xia, Yu-Qi 1 ; Xia, Yang 1 ; Zhang, Meng-Ting 1 ; Ye, Zi 1 ; Sun, Rui-Qing 1 ; Liu, Xiao-Mei 2 ; Pu, Jin-Ji 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Key Lab Integrated Pest Management Trop Grops, Minist Agr & Rural Affairs, Haikou 571101, Peoples R China
2.Hainan Univ, Coll Plant Protect, Haikou 570228, Peoples R China
3.Natl Agr Expt Stn Agr Microbiol, Jiangsu Lixiahe Dist Inst Agr Sci, Yangzhou 225007, Peoples R China
4.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Peoples R China
关键词： filamentous fungus; Colletotrichum gloeosporioides; CRISPR; Cas9 knockout mutants; complementary strain; pathogenicity
期刊名称：AGRONOMY-BASEL （ 影响因子：3.7； 五年影响因子：4.0 ）
ISSN：
年卷期： 2023 年 13 卷 7 期
页码：
收录情况： SCI
摘要： The filamentous fungus Colletotrichum gloeosporioides is the causative agent of one of the most serious diseases that damage plant fruit. In this study, we discovered and experimentally characterized a new gene in Colletotrichum gloeosporioides named CgloRPCYG. The CRISPR/Cas9 knockout mutant and complementary strain of CgloRPCYG were then obtained by polyethylene glycol (PEG)-mediated protoplast transformation to determine the related phenotypes. Compared with the wild-type strain and complementary mutant, the pathogenicity of the CRISPR/Cas9 knockout mutant was significantly decreased, the conidial yield was significantly reduced, and conidial germination was significantly delayed. These data indicate that CgloRPCYG contributes to pathogenicity, conidial yield, and germination in C. gloeosporioides. The successful application of the CRISPR/Cas9 system in C. gloeosporioides also confirms its utility in filamentous fungi for fundamental research and practical application. Furthermore, CgloRPCYG is a potential target gene for use in the development of plant protection technologies, such as spray-induced gene silencing, with the aim of controlling plant anthracnose disease caused by C. gloeosporioides.
- 相关文献
作者其他论文 更多>>
-
Caffeic Acid O-Methyltransferase Gene Family in Mango (Mangifera indica L.) with Transcriptional Analysis under Biotic and Abiotic Stresses and the Role of MiCOMT1 in Salt Tolerance
作者：Wang, Huiliang;Chen, Zhuoli;Zhang, Mengting;Zhang, He;Wang, Huiliang;Chen, Zhuoli;Lei, Chen;Zhang, Mengting;Pu, Jinji;Zhang, He;Chen, Zhuoli;Luo, Ruixiong;Gao, Aiping
关键词：COMT gene family; salt tolerance; transcriptional analysis; transient overexpression
-
Identification of the ERF gene family of Mangifera indica and the defense response of MiERF4 to Xanthomonas campestris pv. mangiferaeindicae
作者：Lei, Chen;Zhang, He;Lei, Chen;Zhang, Mengting;Wang, Huiliang;Zhang, He;Dang, Zhiguo;Zhu, Min;Chen, Yeyuan;Zhu, Min;Chen, Yeyuan
关键词：Mangifera indica; ERF gene family; Expression profile; JA/ETH signaling pathway; Callose; Defense
-
MiMYB10 transcription factor regulates biosynthesis and accumulation of carotenoid involved genes in mango fruit
作者：Dang, Zhiguo;Zhu, Min;Chen, Huarui;Gao, Aiping;Ma, Weihong;Chen, Yeyuan;Dang, Zhiguo;Zhang, He;Zhu, Min;Chen, Yeyuan;Zhang, Ye;Wei, Yunxie
关键词：Mango; MYB10; Transcriptional regulation; Phytoene desaturase gene; Carotenoid; Peel color
-
A vector-free gene interference system using delaminated Mg-Al-lactate layered double hydroxide nanosheets as molecular carriers to intact plant cells
作者：Zhang, He;Li, Xinyu;Yu, Dong;Guan, Junqi;Ding, Hao;Wan, Yinglang;Zhang, He;Wu, Hongyang;Wang, Qiang
关键词：RNA delivery; dsRNA; RNAi; Bioconjugates; Target gene expression
-
The Involvement of the Laccase Gene Cglac13 in Mycelial Growth, Germ Tube Development, and the Pathogenicity of Colletotrichum gloeosporioides from Mangoes
作者：Zhang, Mengting;Xiao, Chunli;Tan, Qing;Dong, Lingling;Liu, Xiaomei;Zhang, He;Zhang, Mengting;Xiao, Chunli;Tan, Qing;Zhang, He;Pu, Jinji
关键词：mango; Colletotrichum gloeosporioides; laccase; germ tube development; pathogenicity
-
Combined Application of Organic Fertilizer with Microbial Inoculum Improved Aggregate Formation and Salt Leaching in a Secondary Salinized Soil
作者：Peng, Yuanyuan;Zhang, He;Lian, Jinshan;Li, Guihua;Zhang, Jianfeng;Zhang, Wen
关键词：secondary salinization; P. brevitarsis larvae frass; B. amyloliticus; T. harziensis; aggregate stability; leaching; plant-growth-promoting microbe
-
Optimized synthesis of layered double hydroxide lactate nanosheets and their biological effects on Arabidopsis seedlings
作者：Wu, Hongyang;Zhang, He;Li, Xinyu;Wan, Yinglang;Wu, Hongyang;Wang, Jiankun;Wan, Yinglang;Wu, Hongyang;Zhang, He;Zhang, Yu;Wang, Qiang
关键词：LDH-lactate-NS; Cytotoxicity; Promote root elongation; Auxin flux
