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Development of a sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for the antimalaria active ingredient artemisinin in the Chinese herb Artemisia annua L.

文献类型： 外文期刊

作者： He, Su-Ping 1 ; Tan, Gui-Yu 1 ; Li, Gang 2 ; Tan, Wei-Ming 1 ; Nan, Tie-Gui 1 ; Wang, Bao-Min 1 ; Li, Zhao-Hu 1 ; Li, Qin 1 ;

作者机构： 1.China Agr Univ, Coll Agron & Biotechnol, Beijing 100193, Peoples R China

2.Jilin Acad Agr Sci, Agr Environm & Resources Res Ctr, Changchun 130124, Peoples R China

3.Univ Hawaii, Dept Mol Biosci & Bioengn, Honolulu, HI 96822 USA

关键词： Malaria;Artemisinin;Artemisia annua;Herb medicine;ELISA;Antibody;PERFORMANCE LIQUID-CHROMATOGRAPHY;LIGHT-SCATTERING DETECTOR;GAS-CHROMATOGRAPHY;ACID;DERIVATIVES;ARTEANNUIN;QINGHAOSU;DRUGS

期刊名称：ANALYTICAL AND BIOANALYTICAL CHEMISTRY （ 影响因子：4.142； 五年影响因子：3.863 ）

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收录情况： SCI

摘要： Artemisinin is an endoperoxide sesquiterpene lactone isolated from the Chinese medicinal plant Artemisia annua L. It has been widely used in South-East Asia and Africa as an effective drug against sensitive and multidrug-resistant Plasmodium falciparum malaria. A monoclonal antibody (mAb), designated as 3H2, was generated with artesunate-bovine serum albumin conjugate as the immunogen. mAb 3H2 was used to develop a highly sensitive and specific indirect competitive enzyme-linked immunosorbent assay (icELISA) for artemisinin. The concentration of analyte producing 50% of inhibition (IC50) and the working range of the icELISA were 1.3 and 0.2-5.8 ng/mL, respectively. The mAb 3H2 recognized the artemisinin analogs artesunate, dihydroartemisinin, and artemether with cross-reactivity of 650%, 57%, and 3%, respectively, but negligibly recognized deoxyartemisinin and the artemisinin precursors arteannuin B and artemisinic acid. The average recoveries of artemisinin fortified in A. annua samples at concentrations from 156 to 5,000 mu g/g determined by icELISA ranged from 91% to 98%. The icELISA was applied for the determination of artemisinin in different wild A. annua samples and the results were confirmed by high-performance liquid chromatography (HPLC) analysis. The correlation coefficient (R-2) between the two assays was larger than 0.99, demonstrating a good agreement between the icELISA and HPLC results. This ELISA is suitable for quality assurance of A. annua L. materials.

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