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Polyethylene degradation mediated by Klebsiella variicola isolated from the gut of insect larvae

文献类型： 外文期刊

作者： Hu, Jing 1 ; Peng, Bowen 1 ; Liu, Zao 1 ; Long, Tong 2 ; Gao, Wa 1 ; Wang, Yongze 1 ; Liu, Xiaonan 1 ; Zhao, Jinfang 1 ;

作者机构： 1.Hubei Univ Technol, Natl Ctr Cellular Regulat & Mol Pharmaceut 111, Sch Life & Hlth Sci,Cooperat Innovat Ctr Ind Ferme, Key Lab Fermentat Engn,Minist Educ & Hubei Prov, Wuhan 430068, Peoples R China

2.Hubei Acad Agr Sci, Natl Biopesticide Engn Technol Res Ctr, Hubei Biopesticide Engn Res Ctr, Wuhan 430064, Peoples R China

关键词： Polyethylene; Biodegradation; Klebsiella variicola ZB-1; CueO; Laccase-like multicopper oxidase; Enzymatic activity

期刊名称：INTERNATIONAL BIODETERIORATION & BIODEGRADATION （ 影响因子：4.1； 五年影响因子：4.3 ）

ISSN： 0964-8305

年卷期： 2025 年 205 卷

页码：

收录情况： SCI

摘要： The biodegradation of polyethylene (PE) presents a promising approach to resolve plastic waste pollution, yet research achievements in this field are relatively scarce. In this study, two PE-degrading strains, Klebsiella variicola ZB-1 and Pseudomonas fulva ZH-2, were isolated from the gut microbiota of Spodoptera frugiperda larvae, and ZB-1 achieved a remarkable 9.79 +/- 1.41 % weight loss of low-density polyethylene (LDPE) within 35 days when fed with PE powder. Scanning electron microscopy and water contact angle measurements revealed that these strains could adhere to PE films and render them hydrophilic. Fourier-transform infrared spectroscopy analysis showed an increase in the oxygen-carbon ratio of the PE films, facilitating degradation. In addition, genome sequencing was utilized to explore the underlying PE degradation mechanisms, and a laccase-like multicopper oxidase (CueO) was demonstrated to have obvious PE degradation capability for the first time. This enzyme exhibited outstanding pH stability (optimal pH 2.5) and temperature tolerance (55 degrees C), with activity further enhanced by Cu2+/Mn2+ ions. Comprehensive characterization using SEM, FTIR, and WCA systematically revealed biofilm-mediated surface erosion and polymer chain destabilization mechanisms. Overall, this study established a novel biological resource library for PE degradation by exploring the genetic information of PEdegrading microorganisms and their key enzyme systems, laying a theoretical foundation for elucidating the molecular mechanisms of polyolefin biodepolymerization.

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