您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Iso-Seq combined with RNA-Seq analysis provided insight into the molecular response to dietary oxidized fish oil in juvenile Amur sturgeon, Acipenser schrenckii

文献类型： 外文期刊

作者： Yu, Huanhuan 1 ; Ren, Yuanyuan 2 ; Xu, Guanling 1 ; Xue, Min 3 ; Xing, Wei 1 ; Li, Tieliang 1 ; Jiang, Na 1 ; Ma, Zhihong 1 ; Luo, Lin 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Fisheries Res Inst, Beijing Key Lab Fishery Biotechnol, Beijing 100068, Peoples R China

2.Chinese Acad Fishery Sci, Key Lab Control Qual & Safety Aquat Prod, Minist Agr & Rural Affairs, Beijing 100141, Peoples R China

3.Chinese Acad Agr Sci, Natl Aquafeed Safety Assessment Ctr, Feed Res Inst, Beijing 100081, Peoples R China

关键词： Amur sturgeon; Transcriptome; Oxidized fish oil; Dysregulation; foxo1; pi3kca

期刊名称：AQUACULTURE （ 影响因子：5.135； 五年影响因子：5.125 ）

ISSN： 0044-8486

年卷期： 2022 年 552 卷

页码：

收录情况： SCI

摘要： Despite the economic and biological importance of Amur sturgeon in the world, limited full-length transcripts are available. Oxidized fish oil (OFO) might induce injury stress, but few related studies were conducted in Amur sturgeon. In this study, an 8-week experiment with diets containing 6% fresh fish oil (FFO) or 6% OFO was conducted, and Iso-Seq combined with RNA-Seq analysis were used to explore the molecular response to dietary OFO in juvenile Amur sturgeon. A total of 18,408 high quality, unique full-length isoforms were generated by Iso-Seq, with an average length of 2180 bp and an N50 of 2793 bp. similar to 382 million clean reads were obtained from RNA-Seq, and the mapping ratio of the clean reads to the unigenes was 84.25% similar to 88.25%. There were 839 differently expressed genes (DEGs) in FFO vs OFO, of which 360 were up-regulated, and 479 were downregulated. DEGs were mapped into 276 KEGG pathways, of which 45 pathways were significantly influenced. The proportion analysis of 45 significantly changed pathways revealed that human disease was the most enriched category, and cancers, endocrine system, signal transduction, cell growth and death and carbohydrate metabolism was the most affected subcategory in each category, indicating that OFO might induce cancers, endocrine disorder, and carbohydrate metabolism disruption. Further analysis discovered that transcriptional misregulation in cancer and FOXO signaling pathway were the top2 regulated pathways. PPI network analysis showed that foxo1 and pik3c alpha were identified as hub genes in response to dietary OFO in Amur sturgeon. The present study provided the first systematic full-length transcriptome dataset of Amur sturgeon and laid a solid foundation for further research on the molecular mechanism in response to dietary OFO.

  • 相关文献

[1]Iso-Seq combined with RNA-Seq analysis provided insight into the molecular response to dietary oxidized fish oil in juvenile Amur sturgeon, Acipenser schrenckii. Yu, Huanhuan,Xu, Guanling,Xing, Wei,Li, Tieliang,Jiang, Na,Ma, Zhihong,Luo, Lin,Ren, Yuanyuan,Xue, Min. 2022

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台