Accelerated and signal amplified nanozyme-based lateral flow assay of acetamiprid based on bivalent triple helix aptamer
文献类型： 外文期刊
作者： Mao, Minxin 1 ; Chen, Xiujin 4 ; Cai, Yina 5 ; Yang, Hanjie 1 ; Zhang, Cunzheng 7 ; Zhang, Yi 1 ; Wang, Zhouping 1 ; Peng, Chifang 1 ;
作者机构： 1.Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China
2.Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Peoples R China
3.Shandong Inst Pomol, Tai An 271018, Peoples R China
4.Henan Univ Sci & Technol, Coll Food & Bioengn, Luoyang 471000, Peoples R China
5.Inspection & Quarantine Technol Ctr, Shenzhen Customs, Shenzhen 518045, Peoples R China
6.Jiangnan Univ, Int Joint Lab Food Safety, Wuxi 214122, Peoples R China
7.Jiangsu Acad Agr Sci, State Key Lab Food Qual & Safety, Nanjing 210014, Peoples R China
8.Jiangnan Univ, Sch Food Sci, Wuxi, Peoples R China
关键词： Aptamer engineering; Lateral flow assay; Acetamiprid; Nanozyme
期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：8.4； 五年影响因子：7.2 ）
ISSN：
年卷期： 2023 年 378 卷
页码：
收录情况： SCI
摘要： The lateral flow assay (LFA) has received extensive attention and been widely used in various targets. However, the development of aptamer based LFA (Apt-LFA) is still far behind, especially for small molecule targets detection. The shortcoming of long response time severely hindered the Apt-LFA strip application in on-site detection of small molecules contaminants in environment and foods. Herein, we introduce bivalent triple helix aptamer (BTHA) and nanozyme yielding an accelerated and signal amplified LFA for acetamiprid detection. We obtained a BTHA with two 10 nt arms, which could shorten the response time between the aptamer and acetamiprid by 6 times, thus dramatically accelerating the detection of acetamiprid. Moreover, we integrate Au@Pt nanozyme to further amplify the detection signal by catalyzing the color development of substrate. In conjunction with smartphone-based analytical device, the Apt-LFA strip enabled the on-site quantitative detection of acetamiprid residue. It is believed that our strategy will facilitate the development of Apt-LFA against small molecule contaminants.
- 相关文献
作者其他论文 更多>>
-
Combined detection of foodborne pathogens in irrigation water in Jiangsu, China
作者：Xie, Yajing;Zhang, Cunzheng;Liu, Xianjin;Xie, Yajing;Lu, Jiayao;Liu, Xianjin;Li, Bin;Fan, Bochao;Li, Dandi;Du, Xuefei;Xie, Yajing;Schmidt, Stefan
关键词：Irrigation water; Foodborne pathogens; Combined detection; Noroviruses; Strawberry production
-
Using designed polypeptides to develop novel phage-mediated triple antibody sandwich immunoassay for the specific detection of Escherichia coli O157:H7 in milk
作者：Xue, Chang;Ouyang, Qin;Zhang, Cunzheng;Xue, Chang;Fu, Jinmei;Tao, Tingting;Zhu, Shouhu;Han, Bingsong;Wang, Yulong;Ye, Yuhui;Zhang, Cunzheng;Fu, Jinmei;Zhang, Cunzheng;Zhu, Shouhu;Zhang, Cunzheng;Hammock, Bruce D.;Hammock, Bruce D.;Luo, Ruifeng
关键词：Polypeptides; Phage-displayed nanobody; Serotype discrimination; Triple antibody sandwich immunoassay; Escherichia coli O157:H7
-
A Novel Camel Milk-Derived Peptide LLPK Improves Glucose-Lipid Metabolism in db/db Mice via PPAR Signaling Pathway
作者：Han, Binsong;Ye, Yuhui;Zhang, Cunzheng;Han, Binsong;Zhang, Lina;Zhou, Peng;Han, Binsong;Zhang, Lina;Zhou, Peng
关键词：camel milk-derived peptide; diabetes; hepatic proteome; PPAR signaling pathway
-
Minimalist nanobody architecture: A double-edged sword for immunological analysis
作者：Wang, Yulong;Liu, Pengyan;Zhang, Yaodong;Zhang, Cunzheng;Wang, Yulong;Luo, Ruifeng;Yang, Huijuan;Dong, Sa;Hua, Xiude;Hammock, Bruce D.
关键词：Nanobody-based immunoassay; Miniaturized structure; Small size; Bioconjugation chemistry
-
Investigation and insights into the technical strategies for reducing agrochemical inputs in rice farming to enhance agroecosystem resilience and food safety, A case study in China
作者：Zhang, Yaodong;Zhang, Haoran;Zhang, Cunzheng;Zhang, Yaodong;Zhang, Haoran;Chen, Jian;Li, Pan;Wang, Yulong;Liu, Pengyan;Zhang, Cunzheng;Tao, Tingting;Zhu, Yiyong;Routledge, Michael N.;Yang, Cuifeng
关键词：Agrochemical; Heavy metal; Metagenomics; Risk assessment; Soil fertility; Sustainable agriculture
-
An intelligent method for detection of small target fungal wheat spores based on an improved YOLOv5 with microscopic images
作者：Ren, Zhizhou;Liang, Kun;Zhang, Yingqi;Song, Jinpeng;Wu, Xiaoxiao;Zhang, Chi;Mei, Xiuming;Zhang, Yi;Liu, Xin
关键词：Wheat; Fungal spore detection; Feature fusion; Attention mechanism; YOLO
-
CVCQ: Off-Chain Committee-Based Verifiable Cross-Chain Query Scheme for Large-Scale BIoT
作者：Yang, Haotian;Zhang, Cunzheng;Yang, Haotian;Zhang, Cunzheng;Zhang, Xiaoyu;Wang, Liangmin;Xie, Zhan;Wang, Liang;Zhang, Xiaoyu;Wang, Liangmin;Xie, Zhan;Wang, Liang;Liu, Lu;Liu, Pengyan
关键词：Aggregation accumulator; blockchain; cross-region; cross-region; IoT; IoT; verifiability; verifiability; verifiability
