Monoacylglycerol lipase from marine Geobacillus sp. showing lysophospholipase activity and its application in efficient soybean oil degumming
文献类型： 外文期刊
作者： Liu, Xuan 1 ; Wang, Weifei 2 ; Zhao, Zexin 3 ; Xu, Long 4 ; Yang, Bo 5 ; Lan, Dongming 1 ; Wang, Yonghua 1 ;
作者机构： 1.South China Univ Technol, Sch Food Sci & Engn, Guangzhou 510640, Peoples R China
2.Guangdong Acad Agr Sci, Minist Agr & Rural Affairs, Sericultural & Argi Food Res Inst, Key Lab Funct Foods,Guangdong Key Lab Agr Prod Pr, 133 Yiheng St, Dongguanzhuang Rd, Tianhe Dist, Guangzhou 510610, Peoples R China
3.Hubei Univ Technol, Minist Educ, Key Lab Fermentat Engn, Wuhan 430068, Peoples R China
4.Henan Agr Univ, Coll Food Sci & Technol, Zhengzhou 450002, Peoples R China
5.South China Univ Technol, Sch Biosci & Bioengn, Guangzhou 510006, Peoples R China
6.Intelligent Biomfg Co Ltd, Guangdong Youmei Inst, Foshan 528200, Guangdong, Peoples R China
关键词： Monoacylglycerol lipase; Lysophospholipase activity; Enzymatic degumming; Lysophospholipids; Glycerophosphocholine phosphodiesterase; activity; UPLC-Q-TOF-MS
期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）
ISSN： 0308-8146
年卷期： 2023 年 406 卷
页码：
收录情况： SCI
摘要： Enzymatic degumming is an essential refining process to improve oil quality. In this study, a monoacylglycerol lipase GMGL was derived from marine Geobacillus sp., and was found that not only took monoacylglycerol (MAG) as substrate, but also had activity toward lysophosphatidylcholine (LPC), lysophosphatidylethanolamine (LPE) and glycerolphosphatidylcholine (GPC). Binding free energy showed LPC and LPE could bind with enzyme stably as MAG. It presented great potential in the field of enzymatic degumming. The phosphorus content in crude soybean oil decreased from 680.50 to 2.01 mg/kg and the yield of oil reached to 98.80 % after treating with phospholipase A1 (Lecitase Ultra) combined with lipase GMGL. An ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was developed to identify 21 differential phospholipids between crude soybean oil and enzymatic treatment. This work might shed some light on understanding the catalytic mechanism of monoacylglycerol lipase and provide an effective strategy for enzymatic degumming.
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