Construction of biocatalysts based on P450BM3 for the degradation of non-steroidal anti-inflammatory drugs
文献类型： 外文期刊
作者： Yang, Yadan 1 ; Zhang, Weikang 1 ; Wang, Fang 1 ; Li, Dong 1 ; Meng, Xiangmin 2 ; Sun, Xiaochun 3 ; Xu, Jiakun 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, State Key Lab Mariculture Biobreeding & Sustainabl, Lab Marine Drugs & Byprod Pilot Natl Lab Marine Sc, Qingdao 266071, Peoples R China
2.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Qingdao 266042, Peoples R China
3.Natl Oceanog Ctr Qingdao, Marine Sci Res Inst Shandong Prov, Qingdao 266104, Peoples R China
4.Minist Agr & Rural Affairs, Key Lab Sustainable Dev Polar Fisheries, Qingdao 266071, Peoples R China
关键词： NSAID; P450BM3; Rational design; Biocatalysis; Degradation mechanism
期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：11.3； 五年影响因子：12.4 ）
ISSN： 0304-3894
年卷期： 2024 年 480 卷
页码：
收录情况： SCI
摘要： Non-steroidal anti-inflammatory drugs (NSAIDs) are widespread pollutants in aquatic environments, posing significant risks to both ecosystems and human health due to their persistence and bioaccumulation. Effective and sustainable degradation methods are urgently required to address this environmental challenge. This study aims to design and optimize a cytochrome P450BM3-based biocatalyst for the rapid and efficient degradation of NSAIDs by direct chemical intervention and protein engineering. The novel biocatalyst achieved efficient biodegradation of four common NSAIDs. Notably, the F87I/T268D mutant achieved 99.22 % degradation of diclofenac (DCF) within 10 min, and degraded meloxicam (MEL) and phenylbutazone (PBZ) at rates of 98.86 % and 90.51 % within 5 min, respectively. Furthermore, the F87G mutant accomplished 99.08 % degradation of acetaminophen (APAP) within just 2 min. The catalytic properties of P450BM3 and its mutants were evaluated
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