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A single nucleotide polymorphism (SNP) marker linked to the fragrance gene in rice (Oryza sativa L.)

文献类型： 外文期刊

作者： Jin, QS 1 ; Waters, D 2 ; Cordeiro, GM 2 ; Henry, RJ 2 ; Reinke, RF 2 ;

作者机构： 1.So Cross Univ, Ctr Plant Conservat Genet, Lismore, NSW 2480, Australia

2.So Cross Univ, Ctr Plant Conservat Genet, Lismore, NSW 2480, Australia; Zhejiang Acad Agr Sci, Crop Res Inst, Hangzhou 310021, Peoples R China; Yanco Agr Inst, Yanco, NSW 2703, Australia

关键词： Single nucleotide polymorphism;Rice;Oryza sativa;Fragrance;Marker-assited selection;Blast resistance gene;Scent;Inheritance;Sequence;Aroma;Population

期刊名称：PLANT SCIENCE （ 影响因子：4.729； 五年影响因子：5.132 ）

ISSN：

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收录情况： SCI

摘要： The whole rice genome sequence was used to assist in the identification of a single nucleotide polymorphism (SNP) marker linked to the fragrance gene (fgr) in rice. Genes flanked by restriction fragment length polymorphism and microsatellite markers known to be linked to the fragrance gene were identified by DNA sequence alignment of EST sequences against BAC clones covering this region of chromosome eight. Re-sequencing and comparison of parts of these genes derived from a fragrant and a non-fragrant cultivar revealed only one SNP (a C/T transition) in more than 6 kbp of sequence from 14 genes. Ten of eleven fragrant genotypes and six of 14 non-fragrant genotypes tested carried the C allele. This approach indicated a generally low level of SNP polymorphism in cultivated rice suggesting that association of SNP with phenotypes should be an efficient path to gene discovery in cultivated rice. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved. [References: 25]

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