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A perfect marker for fragrance genotyping in rice

文献类型: 外文期刊

作者: Bradbury, LMT 1 ; Henry, RJ 2 ; Jin, QS 2 ; Reinke, RF 2 ; Waters, DLE 3 ;

作者机构: 1.So Cross Univ, Ctr Plant Conservat Genet, Lismore, NSW 2480, Australia

2.So Cross Univ, Ctr Plant Conservat Genet, Lismore, NSW 2480, Australia; So Cross Univ, Grain Foods CRC, Lismore, NSW 2480, Australia; Zhejiang Acad Agr Sci, Crop Res Inst, Hangzhou, Peoples R China; Yanco Agr Inst, Yanco, NSW 2703, Australia

3.So Cross Univ, Ctr Plant Conservat Genet, Lismore, NSW 2480, Australia; So Cross Univ, Grain Foods CRC, Lismore, NSW 2480, Australia; Zhejiang Acad Agr Sci, Crop Res Inst, Hangzhou, Peoples R China; Yanco Agr Inst, Yanco, N

关键词: aromatic;assay;basmati;high-throughput;jasmine;Oryza sativa

期刊名称:MOLECULAR BREEDING ( 影响因子:2.589; 五年影响因子:2.75 )

ISSN: 1380-3743

年卷期: 2005 年 16 卷 4 期

页码:

收录情况: SCI

摘要: Allele specific amplification (ASA) is a low-cost, robust technique that can be utilised to discriminate between alleles that differ by SNP's, insertions or deletions, within a single PCR tube. Fragrance in rice, a recessive trait, has been shown to be due to an eight bp deletion and three SNP's in a gene on chromosome 8 which encodes a putative betaine aldehyde dehydrogenase 2 (BAD2). Here we report a single tube ASA assay which allows discrimination between fragrant and non-fragrant rice varieties and identifies homozygous fragrant, homozygous non-fragrant and heterozygous non-fragrant individuals in a population segregating for fragrance. External primers generate a fragment of approximately 580 bp as a positive control for each sample. Internal and corresponding external primers produce a 355 bp fragment from a non-fragrant allele and a 257 bp fragment from a fragrant allele, allowing simple analysis on agarose gels.

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