AmiRNA Technology Enhances Tomato Disease Resistance by Suppressing Plant-Pathogen Interaction Pathways through Inhibiting TYLCV Replication
文献类型： 外文期刊
作者： Wang, Xian 1 ; Wang, Baoqiang 2 ; Jin, Baoxia 2 ; Wang, Weijie 2 ; Zhu, Xiaolin 2 ; Liu, Wenyu 5 ; Yang, Ling 6 ; Wei, Xiaohong 1 ;
作者机构： 1.Gansu Agr Univ, Agron Coll, Lanzhou 730070, Peoples R China
2.Gansu Prov Key Lab Aridland Crop Sci, Lanzhou 730070, Peoples R China
3.Gansu Key Lab Crop Genet & Germplasm Enhancement, Lanzhou 730070, Peoples R China
4.Gansu Agr Univ, Coll Life Sci & Technol, Lanzhou 730070, Peoples R China
5.Gansu Acad Agr Sci, Lanzhou 730070, Peoples R China
6.Lanzhou Jiaotong Univ, Sch Biol & Pharmaceut Engn, Lanzhou 730070, Peoples R China
关键词： amiRNA technology; tomato; TYLCV; plant-pathogen interaction
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）
ISSN： 0021-8561
年卷期： 2024 年 72 卷 47 期
页码：
收录情况： SCI
摘要： Tomato yellow leaf curl virus disease has seriously threatened the quality and yield of tomatoes. In this study, we investigated the role of amiRNA technology in disease resistance in tomatoes (cherry tomato and large-fruited tomato) and analyzed the physiological and molecular mechanisms of disease resistance in transgenic plants. TYLCV contains six functional genes, of which the C1, C2, and V1 genes have more phosphorylation sites and glycosylation sites, and the protein structure is more complex. The virus replication was inhibited, the peroxidation of membrane lipids was reduced, and disease resistance was enhanced in all transgenic cherry tomato (J6) plants in which the C1, C2, and V1 genes were silenced, respectively. Similarly, silencing of the C1 gene enhanced disease resistance in large-fruited tomatoes. In conclusion, amiRNA technology hinders viral replication, leading to reduced activity of the tomato plant-pathogen interaction pathway and weakening tomato-virus interactions, thereby improving disease resistance.
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