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Fads2b Plays a Dominant Role in increment 6/ increment 5 Desaturation Activities Compared with Fads2a in Common Carp (Cyprinus carpio)

文献类型： 外文期刊

作者： Zhao, Ran 1 ; Yang, Chen-Ru 1 ; Wang, Ya-Xin 1 ; Xu, Zi-Ming 1 ; Li, Shang-Qi 1 ; Li, Jin-Cheng 1 ; Sun, Xiao-Qing 1 ; Wang, Hong-Wei 1 ; Wang, Qi 1 ; Zhang, Yan 1 ; Li, Jiong-Tang 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Key Lab Aquat Genom, Minist Agr & Rural Affairs, Beijing 100141, Peoples R China; Chinese Acad Fishery Sci, Beijing Key Lab Fishery Biotechnol, Beijing 100141, Peoples R China

关键词： common carp; fatty acid desaturase 2; highly unsaturated fatty acids; desaturation activity; dominant expression

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）

ISSN： 1661-6596

年卷期： 2023 年 24 卷 13 期

页码：

收录情况： SCI

摘要： Highly unsaturated fatty acids (HUFAs) are essential for mammalian health, development and growth. However, most mammals, including humans, are incapable of synthesizing n-6 and n-3 HUFAs. Fish can convert C18 unsaturated fatty acids into n-6 and n-3 HUFAs via fatty acid desaturase (Fads), in which Fads2 is a key enzyme in HUFA biosynthesis. The allo-tetraploid common carp theoretically encode two duplicated fads2 genes. The expression patterns and desaturase functions of these two homologous genes are still unknown. In this study, the full length of the fads2a and fads2b were identified in common carp (Cyprinus carpio). Expression analyses indicate that both genes were mainly expressed in the liver and the expression of fads2b is higher than fads2a at different developmental stages in carp embryos. Heterogenous expression and 3D docking analyses suggested that Fads2b demonstrated stronger increment 6 and increment 5 desaturase activities than Fads2a. The core promotor regions of fads2a and fads2b were characterized and found to have different potential transcriptional binding sites. These results revealed the same desaturase functions, but different activities of two homologues of fasd2 genes in common carp. The data showed that fads2b played a more important role in HUFA synthesis through both expression and functional analyses.

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