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BnaCRCs with domestication preference positively correlate with the seed-setting rate of canola

文献类型： 外文期刊

作者： Qin, Pei 1 ; Gao, Jinxiang 1 ; Shen, Wenhao 1 ; Wu, Zengxiang 2 ; Dai, Cheng 1 ; Wen, Jing 1 ; Yi, Bin 1 ; Ma, Chaozhi 1 ; Shen, Jinxiong 1 ; Fu, Tingdong 1 ; Tu, Jinxing 1 ;

作者机构： 1.Huazhong Agr Univ, Natl Ctr Rapeseed Improvement Wuhan, Coll Plant Sci & Technol, Hubei Hongshan Lab,Natl Key Lab Crop Genet Improv, Wuhan 430070, Peoples R China

2.Guangdong Acad Agr Sci, Vegetable Res Inst, Guangdong Key Lab New Technol Res Vegetables, Guangzhou 510640, Guangdong, Peoples R China

关键词： Brassica napus; seed-setting rate; CRABS CLAW; domestication; carpel development; nectary; stigma papilla cell; map-based cloning

期刊名称：PLANT JOURNAL （ 影响因子：7.091； 五年影响因子：8.028 ）

ISSN： 0960-7412

年卷期：

页码：

收录情况： SCI

摘要： Canola (Brassica napus) is an important oil crop worldwide. The seed-setting rate (SS) is a critical factor in determining its yield, and the development of pistils affects pollination and seed sets. However, research on seed-setting defects has been limited owing to difficulties in the identification of phenotypes, mutations, and complex genetic mechanisms. In this study, we found a stigma defect (sd) mutant in B. napus, which had no nectary. The SS of sd mutants in the field was approximately 93.4% lower than that of the wild type. Scanning and transmission electron microscopy imaging of sd mutants showed a low density of stigma papillary cells and stigma papillary cell vacuoles that disappeared 16 h after flowering. Genetic analysis of segregated populations showed that two recessive nuclear genes are responsible for the mutant phenotype of sd. Based on re-sequencing and map-based cloning, we reduced the candidate sites on ChrA07 (BnaSSA07) and ChrC06 (BnaSSC06) to 30 and 67 kb, including six and eight predicted genes, respectively. Gene analyses showed that a pair of CRABS CLAW (CRC) homeologous genes at BnaSSA07 and BnaSSC06 were associated with the development of carpel and nectary. BnaSSA07.CRC and BnaSSC06.CRC candidate genes were found to be expressed in flower organs only, with significant differences in their expression in the pistils of the near-isogenic lines. DNA sequencing showed transposon insertions in the upstream region and intron of the candidate gene BnaSSA07.crc. We also found that BnaSSC06.crc exists widely in the natural population and we give possible reasons for its widespread existence.

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