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Bioinformatics and Transcriptome Analysis of CFEM Proteins in Fusarium graminearum

文献类型： 外文期刊

作者： Chen, Lingqiao 1 ; Wang, Haoyu 1 ; Yang, Junhua 2 ; Yang, Xianli 2 ; Zhang, Mengyuan 1 ; Zhao, Zhihui 2 ; Fan, Yingyin 1 ;

作者机构： 1.Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai 201306, Peoples R China

2.Shanghai Acad Agr Sci, Lab Qual & Safety Risk Assessment Agroprod Shangh, Shanghai Key Lab Protected Hort Technol, Minist Agr,Inst Agrofood Stand & Testing Technol, 1000 Jinqi Rd, Shanghai 201403, Peoples R China

3.Xinjiang Acad Agr Sci, Inst Quanl Stand & Testing Technol Agroprod, Urumqi 830091, Peoples R China

关键词： Fusarium graminearum; CFEM domain; candidate effectors; bioinformatics analysis; transcriptional analysis

期刊名称：JOURNAL OF FUNGI （ 影响因子：5.816； 五年影响因子：6.499 ）

ISSN：

年卷期： 2021 年 7 卷 10 期

页码：

收录情况： SCI

摘要： Fusarium blight of wheat is usually caused by Fusarium graminearum, and the pathogenic fungi will secrete effectors into the host plant tissue to affect its normal physiological process, so as to make it pathogenic. The CFEM (Common in Fungal Extracellular Membrane) protein domain is unique to fungi, but it is not found in all fungi. The CFEM protein contained in F. graminearum may be closely related to pathogenicity. In this study, 23 FgCFEM proteins were identified from the F. graminearum genome. Then, features of these proteins, such as signal peptide, subcellular localization, and transmembrane domains, etc., were analyzed and candidate effectors were screened out. Sequence alignment results revealed that each FgCFEM protein contains one CFEM domain. The amino acids of the CFEM domain are highly conserved and contain eight spaced cysteines, with the exception that FgCFEM8, 9, and 15 lack two cysteines and three cysteines were missed in FgCFEM18 and FgCFEM22. A recently identified CFEM_DR motif was detected in 11 FgCFEMs, and importantly we identified two new conserved motifs containing about 29 and 18 amino acids (CFEM_WR and CFEM_KF), respectively, in some of FgCFEM proteins. Transcriptome analysis of the genes encoding CFEM proteins indicated that all the CFEM-containing genes were expressed during wheat infection, with seven and six genes significantly up- and down-regulated, respectively, compared with in planta and in vitro. Based on the above analysis, FgCFEM11 and FgCFEM23 were predicted to be F. graminearum effectors. This study provides the basis for future functional analyses of CFEM proteins in F. graminearum.

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