文献类型： 外文期刊

作者： Arif, Muhammad ¹ ; ul Islam, Saif ¹ ; Alotaibi, Saqer S. ³ ; Elshehawi, Ahmed M. ³ ; Ahmed, Mohamed A. A. ⁴ ; Al-Sa ¹ ;

作者机构： 1.Fujian Agr & Forestry Univ, Dept Plant Protect, Fuzhou, Peoples R China

2.Guangdong Acad Agr Sci, Vegetable Res Inst, Guangzhou, Peoples R China

3.Taif Univ, Dept Biotechnol, Coll Sci, At Taif, Saudi Arabia

4.Alexandria Univ, Fac Agr Saba Basha, Plant Prod Dept Hort Med & Aromat Plants, Alexandria, Egypt

5.Sultan Qaboos Univ, Dept Plant Sci, Coll Agr & Marine Sci, Alkhound, Oman

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2021 年 16 卷 5 期

页码：

收录情况： SCI

摘要： Geminiviruses are insect-transmissible, economically vital group of plant viruses, which cause significant losses to crop production and ornamental plants across the world. During this study, infectious clones of three devastating begomoviruses, i.e., Cotton leaf curl Multan virus (CLCuMuV), Ramie mosaic virus (RamV) and Corchorus yellow vein Vietnam virus (CoYVV) were constructed by following novel protocol. All infectious clones were confirmed by cloning and sequencing. All of the infectious clones were agro-inoculated in Agrobacterium. After the agro-infiltrations, all clones were injected into Nicotiana benthamiana and jute plants under controlled condition. After 28 days of inoculation, plants exhibited typical symptoms of their corresponding viruses. All the symptomatic and asymptomatic leaves were collected from inoculated plants for further analysis. The southern blot analysis was used to confirm the infection of studied begomoviruses. At the end, all the products were sequenced and analyzed.