Genomic and Transcriptomic Dissection of the Large-Effect Loci Controlling Drought-Responsive Agronomic Traits in Wheat
文献类型： 外文期刊
作者： Cao, Liangjing 1 ; Lyu, Mingjie 2 ; Wang, Jingyi 2 ; Wang, Xuan 4 ; Li, Xinchang 2 ; Jing, Ruilian 2 ; Liu, Jun 2 ; Hu, Xinwen 1 ;
作者机构： 1.Hainan Univ, Coll Trop Crops, Haikou 570228, Hainan, Peoples R China
2.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Resources & Genet Improvement, Beijing 100081, Peoples R China
3.Tianjin Acad Agr Sci, Inst Crop Germplasm & Biotechnol, Tianjin 300380, Peoples R China
4.Xian Jiaotong Liverpool Univ, Dept Biol Sci, Suzhou 215123, Peoples R China
关键词： wheat; drought; whole-genome re-sequencing; RNA-sequencing; QTL
期刊名称：AGRONOMY-BASEL （ 影响因子：3.949； 五年影响因子：4.117 ）
ISSN：
年卷期： 2022 年 12 卷 6 期
页码：
收录情况： SCI
摘要： Drought tolerance is one of the most important targets for wheat breeding. Previous population genetics studies have uncovered 20 large-effect quantitative trait loci (QTLs) that contribute to stress-responsive agronomic traits. Here, we identified 19,035,814 single nucleotide polymorphisms and 719,049 insertion/deletion variations in the genomes of two popular winter wheat cultivars, Lu-Mai 14 and Han-Xuan 10, using a whole-genome re-sequencing assay. There were 4972 loss-offunction mutations carried by protein-coding genes, such as CCAI/LHY, AGOI, ABI3/VPI, EIN3, TPP, and ARFs. We carried out a time-course abscisic acid (ABA)-treatment experiment and profiled 61,251 expressed genes in the roots using a strand-specific RNA sequencing approach. A large number of genes showed time-point specific and/or cultivar-preferential responsive expression patterns. Gene ontology enrichment analysis revealed that ABA-responsive genes were associated with stress-related functions. Among the 20 QTLs, we uncovered 306 expressed genes with high- and/or moderateeffect variations and 472 differentially expressed genes. Detailed analysis and verification of the homozygous genomic variations in the candidate genes encoding sulfotransferase, proteinase, kinase, nitrate transporter, and transcription factors suggested previously unexpected pathways associated with abiotic stress responses in wheat.
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