文献类型： 外文期刊
作者： Zhang, Qiang 1 ; Zhang, Yan 2 ; Chai, Yiping 2 ;
作者机构： 1.Shandong Agr Univ, Coll Plant Protect, Tai An, Peoples R China
2.Jiangsu Acad Agr Sci, Nanjing, Jiangsu, Peoples R China
期刊名称：PLOS ONE （ 影响因子：3.752； 五年影响因子：4.069 ）
ISSN： 1932-6203
年卷期： 2022 年 17 卷 3 期
页码：
收录情况： SCI
摘要： CRISPR/LbCas12a system (LbCpf1) has been widely used for genome modification including plant species. However, the efficiency of CRISPR/LbCas12a varied considerably in different plant species and tissues, and the editing efficiency needs to be further improved. In this study, we tried to improve the editing efficiency of CRISPR/LbCas12a in Arabidopsis by optimizing the crRNA expression strategies and Pol II promoters. Notably, the combination of tRNA-crRNA fusion strategy and RPS5A promoter in CRISPR/LbCas12a system has highest editing efficiency, while CRISPR/LbCas12a driven by EC1f-in(crR)p had the highest ratio of homozygous & bi-allelic mutants. In addition, all homozygous & bi-allelic mutants can be stably inherited to the next generation and have no phenotypic separation. In this study, the editing efficiency of the CRISPR/LbCas12a system was improved by selecting the optimal crRNA expression strategies and promoter of LbCas12a in Arabidopsis, which will prove useful for optimization of CRISPR/LbCas12a methods in other plants.
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