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Functional analysis of a PISTILLATA-like gene CcMADS20 involved in floral organs specification in citrus

文献类型： 外文期刊

作者： Hou, Xiao-Jin 1 ; Ye, Li-Xia 1 ; Ai, Xiao-Yan 3 ; Hu, Chun-Gen 1 ; Cheng, Zhong-Ping 4 ; Zhang, Jin-Zhi 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Hort & Forestry Sci, Key Lab Hort Plant Biol, Minist Educ, Wuhan 430070, Peoples R China

2.Henan Inst Sci & Technol, Sch Hort & Landscape Architecture, Xinxiang 453003, Henan, Peoples R China

3.Hubei Acad Agr Sci, Inst Pomol & Tea, Wuhan 430070, Peoples R China

4.Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430070, Hubei, Peoples R China

关键词： Arabidopsis; APETALA3; Citrus; CcMADS20; CcMADS20 promoter

期刊名称：PLANT SCIENCE （ 影响因子：5.363； 五年影响因子：5.454 ）

ISSN： 0168-9452

年卷期： 2022 年 319 卷

页码：

收录情况： SCI

摘要： PISTILLATA (PI), as a member of MADS-box transcription factor, plays an important role in petal and stamen specification in Arabidopsis. However, little is known about PI-like genes in citrus. To understand the molecular mechanism of PI during the developmental process of citrus flower, a PI-like gene CcMADS20 was isolated from Citrus Clemantina. Sequence alignment and phylogenetic analysis revealed that CcMADS20 had relatively high similarity with PI-like homolog and was classified in the core dicotyledonous group. The temporal and spatial expression analyses showed that CcMADS20 was specifically expressed in petal and stamen of citrus flower, which was consistent with PI expression pattern in Arabidopsis. Protein interaction revealed that CcMADS20 could form heterodimer with AP3-like proteins. Furthermore, ectopic overexpression of CcMADS20 in Arabidopsis resulted in transformation of sepals into petal-like structure, as observed in other plants overexpressing a functional PI-like homolog. Additionally, promoter fragments of CcMADS20 were also cloned in the representative 21 citrus varieties. Interestingly, four types of promoters were discovered in these citrus varieties, resulting from two stable insert/deletion fragments (Locus1 and Locus2). The homo/hetero-zygosity of promoter alleles in each variety was strongly related to the evolutionary origin of citrus. Four promoters activity analysis indicated that Locus1 presence inhibited CcMADS20 transcriptional activity and Locus2 presence promoted its transcriptional activity. These findings suggested that CcMADS20 determines petal and stamen development during the evolutionary process of citrus and four promoters discovered, as effective genetic markers, are valuable for citrus breeding practices.

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