Fc gamma receptor-mediated phagocytosis pathway was involved in phagocytosis of mIgNA(+) B lymphocytes from largemouth bass (Micropterus salmoides)
文献类型： 外文期刊
作者： Wu, Jing 1 ; Nie, Yifan 1 ; Wang, Jingya 2 ; Feng, Guoqing 1 ; Hao, Le 1 ; Ma, Yanping 1 ; Li, Yugu 2 ; Liu, Zhenxing 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou 510640, Peoples R China
2.South China Agr Univ, Coll Vet Med, Guangzhou 510642, Peoples R China
3.Key Lab Livestock Dis Prevent Guangdong Prov, Guangzhou, Peoples R China
4.Minist Agr & Rural Affairs, Sci Observat & Expt Stn Vet Drugs & Diagnost Tech, Guangzhou, Peoples R China
5.Collaborat Innovat Ctr GDAAS, Nanjing, Peoples R China
关键词： Fc gamma receptor-mediated phagocytosis; innate immunology; Micropterus solmoides; mIgM(+) B lymphocytes; mRNA-seq
期刊名称：JOURNAL OF FISH BIOLOGY （ 影响因子：2.504； 五年影响因子：2.541 ）
ISSN： 0022-1112
年卷期：
页码：
收录情况： SCI
摘要： The potential for phagocytosis has been proven in teleost B cells, but the research on the regulatory mechanism of phagocytosis remains lacking. In this study, three largemouth bass (Micropterus salmoides) (15 +/- 5 g) were injected intraperitoneally with Nocardia seriolae (10(5) CFU/100 mu l/fish) in vivo, and their spleen was collected at 72 h post-infection for mRNA-seq. After the de novo assembly of the paired-end reads, 73,622 unigenes were obtained. Gene expression profiling revealed that 2043 unigenes were differentially expressed after N. seriolae infection, comprising 1285 upregulated and 758 downregulated unigenes (q-value <0.05, log2FC > vertical bar 2 vertical bar) of which 181 genes were involved in phagocytosis. The Kyoto Encyclopaedia of Genes and Genomes (KEGG) analysis demonstrated that 12 differentially expressed genes (DEG) associated with phagocytosis were enriched in the Fc gamma receptor-mediated phagocytosis signalling pathway. In vitro, the phagocytic ability of mIgM(+) B lymphocytes was validated using indirect immunofluorescence assay (IIFA) and fluorescence activating cell sorter (FACS), and the phagocytosis rates of the mIgM(+) B lymphocytes incubated with a Lyn inhibitor had decreased from 18.533 +/- 6.00% to 11.610 +/- 4.236% compared with the unblocked group. These results suggested that the Fc gamma receptor-mediated phagocytosis signalling pathway had participated in the phagocytosis of B cells and provide further insight into the role of B cells in innate immunology.
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