文献类型： 外文期刊

作者： Wang, Xiaowei ¹ ; Zhou, Xiaonan ¹ ; Li, Chenglong ¹ ; Qu, Chang ¹ ; Shi, Yuangang ¹ ; Li, Cong-Jun ³ ; Kang, Xiaolong ¹ ;

作者机构： 1.Ningxia Univ, Coll Anim Sci & Technol, Key Lab Ruminant Mol & Cellular Breeding, Yinchuan 750021, Peoples R China

2.Inst Anim Sci, Ningxia Acad Agr & Forestry Sci, Yinchuan 750002, Ningxia, Peoples R China

3.ARS, Anim Genom & Improvement Lab, Henry A Wallace Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA

关键词： Bovine skeletal muscle satellite cells; Butyrate; DNA methylation; Transcriptome; DNA methyltransferases; Cell differentiation; Demethylases

期刊名称：GENOMICS （ 影响因子：3.0； 五年影响因子：3.3 ）

ISSN： 0888-7543

年卷期： 2024 年 116 卷 6 期

页码：

收录情况： SCI

摘要： Butyric acid as a short-chain fatty acid (SCFA) is one of the key microbial metabolites of ruminants. Numerous studies indicate that butyrate is crucial in muscle growth and development, and plays an important molecular regulatory role mainly by inhibiting histone deacetylation. DNA methylation, a major epigenetic modification, is involved in cell differentiation. Butyrate, in addition to its role in acetylation modifications, can alter the DNA methylation status of cells. However, the impact of butyrate on the DNA methylation of bovine skeletal muscle satellite cells (SMSCs) remains unclear. In this study, we developed a differentiation model of SMSCs and employed RNA sequencing (RNA-seq) alongside whole genome bisulfite sequencing (WGBS) to explore the effects of butyrate treatment on DNA methylation status and its relationship with gene expression. Treatment of SMSCs with sodium butyrate (NaB) at 1.0 mM for 2 days significantly inhibited the expression of DNA methyltransferases (DNMT1, DNMT2, DNMT3A) at the mRNA and protein levels while promoting the expression of demethylases (TET1, TET2, TET3) at mRNA levels. WGBS identified 4292 differentially methylated regions (DMRs), comprising 2294 hypermethylated and 1998 hypomethylated regions. These DMRs were significantly enriched in the MAPK, cAMP, and Wnt signaling pathways, all of which are implicated in myogenesis and development. Combining RNA-seq and WGBS data revealed a total of 130 overlapping genes, including MDFIC, CREBBP, DMD, LTBP2 and KLF4. These genes are predominantly involved in regulating the FoxO, MAPK, PI3KAkt, and Wnt signaling pathways. This study provides new insights into the effects of butyrate-mediated DNA methylation on SMSC development and enhances our understanding of butyrate as an epigenetic modifier beyond its role in acetylation.