Whole-transcriptome RNA sequencing reveals changes in amino acid metabolism induced in harvested broccoli by red LED irradiation
文献类型： 外文期刊
作者： Yan, Zhicheng 1 ; Xu, Dongying 1 ; Yue, Xiaozhen 1 ; Yuan, Shuzhi 1 ; Shi, Junyan 1 ; Gao, Lipu 1 ; Wu, Caie 2 ; Zuo, Jinhua 1 ; Wang, Qing 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Agrifood Proc & Nutr, Beijing Vegetable Res Ctr, Beijing Key Lab Fruits & Vegetable Storage & Proc,, Beijing 100097, Peoples R China
2.Nanjing Forestry Univ, Coll Light Ind & Food Engn, Nanjing 210037, Jiangsu, Peoples R China
关键词： Whole-transcriptome; Broccoli; LED; Storage; Amino acid
期刊名称：FOOD RESEARCH INTERNATIONAL （ 影响因子：8.1； 五年影响因子：7.7 ）
ISSN： 0963-9969
年卷期： 2023 年 169 卷
页码：
收录情况： SCI
摘要： Whole-transcriptomic profiling combined with amino acid analysis were conducted in order to gain a better understanding of global changes in amino acid metabolism induced in broccoli by red LED irradiation. The results showed that the contents of almost all 16 amino acids in postharvest broccoli were maintained under red LED illumination. The red LED irradiation enhanced the anabolism of amino acid, including the biosynthesis of aromatic amino acids by upregulating the genes' expression in the shikimate pathway, as well as by upregulating the genes' expression which encoding biosynthetic enzymes in the branched-chain amino acid biosynthesis pathway. Red LED irradiation induced the expression of genes encoding aspartate aminotransferase, which plays a role in Asp synthesis, aspartate kinase, which functions in aspartate metabolism, and a cytoplasmic aspartate aminotransferase that converts 2-Oxoglutarate into Glu. Genes encoding imidazole glycerol-phosphate synthase and histidinol-phosphatase, which function in the His biosynthesis pathway, were also upregulated. According to our results, red LED irradiation delays broccoli's yellowing and senescence by regulating amino acid metabolism. These results enhance our understanding of the role of amino acid metabolism in the senescence of broccoli and the mechanism of red LED irradiation to alter amino acid metabolism in harvested broccoli.
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